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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1997-4-8
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pubmed:abstractText |
Rat liver Kupffer cell membranes contain a protein that recognizes specifically oxidized low-density lipoproteins (oxLDL). Visualization after blotting under reducing conditions indicates that the receptor is a monomeric protein, with an estimated molecular mass of 115-120 kDa. N-Glycosidase F and endoglycosidase F treatment resulted in a fall in estimated molecular mass of 24 and 11 kDa respectively, whereas O-glycosidase was ineffective. No effect on the extent of interaction with oxLDL was noticed, suggesting that glycans are not essential for ligand recognition. Using a polyclonal antibody to mouse macrosialin, we visualized macrosialin on blot, and compared this glycoprotein with the oxLDL-binding protein. It appears that the two glycoproteins have a similar molecular mass and are comparably affected by treatment with the different glycosidases. Incubation with trypsin resulted in a reduction in the estimated molecular mass of about 25 kDa for both the oxLDL-binding protein and macrosialin. These results indicate that the oxLDL-binding protein and macrosialin are identical, suggesting a role for macrosialin in modified LDL catabolism.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9065757-13566175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9065757-1429595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9065757-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9065757-165752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9065757-177845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9065757-1919437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9065757-1989982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9065757-2130945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9065757-218198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9065757-2300204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9065757-2606943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9065757-2914924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9065757-3011509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9065757-3194423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9065757-3312248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9065757-3598395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9065757-4335139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9065757-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9065757-6587396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9065757-7520436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9065757-7533292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9065757-7568176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9065757-7685021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9065757-7877989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9065757-8159736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9065757-8288634
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/CD68 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoside Hydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/OLR1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, LDL,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Oxidized LDL,
http://linkedlifedata.com/resource/pubmed/chemical/Scavenger Receptors, Class E,
http://linkedlifedata.com/resource/pubmed/chemical/Trypsin
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
322 ( Pt 2)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
411-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9065757-Animals,
pubmed-meshheading:9065757-Antigens, CD,
pubmed-meshheading:9065757-Antigens, Differentiation, Myelomonocytic,
pubmed-meshheading:9065757-Cross Reactions,
pubmed-meshheading:9065757-Glycoside Hydrolases,
pubmed-meshheading:9065757-Humans,
pubmed-meshheading:9065757-Immunoblotting,
pubmed-meshheading:9065757-Kupffer Cells,
pubmed-meshheading:9065757-Liver,
pubmed-meshheading:9065757-Male,
pubmed-meshheading:9065757-Membrane Glycoproteins,
pubmed-meshheading:9065757-Mice,
pubmed-meshheading:9065757-Oxidation-Reduction,
pubmed-meshheading:9065757-Rats,
pubmed-meshheading:9065757-Rats, Wistar,
pubmed-meshheading:9065757-Receptors, LDL,
pubmed-meshheading:9065757-Receptors, Oxidized LDL,
pubmed-meshheading:9065757-Scavenger Receptors, Class E,
pubmed-meshheading:9065757-Trypsin
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pubmed:year |
1997
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pubmed:articleTitle |
Characterization of a receptor for oxidized low-density lipoproteins on rat Kupffer cells: similarity to macrosialin.
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pubmed:affiliation |
Division of Biopharmaceutics, Leiden-Amsterdam Center for Drug Research, Sylvius Laboratories, The Netherlands.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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