Linked Life Data

9065034

Source:http://linkedlifedata.com/resource/pubmed/id/9065034

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1997-3-17
pubmed:abstractText
The antigen receptor of the MALT lymphoma cells provides crucial information, which may help to elucidate the pathogenesis of that tumor. Therefore hybridomas were produced from five MALT type lymphomas. The identity was proven by sequencing the VH chain of the tumor and the hybridomas. It could be shown the one step of subcloning and limiting dilution is necessary to obtain a monoclonal hybridoma in most cases. So it is indispensable to check the identity of the tumor antibody and the hybridoma antibody for each hybridoma. Otherwise it may be possible that bystander cells infiltrating the tumor, but not belonging to the malignant clone, get fused. Furthermore we provide evidence of an intra tumoral diversity within the MALT type lymphoma that was reflected by the different mutation pattern of the tumor derived hybridomas. Therefore the hybridomas are allowing investigation on the protein level about the hypermutation of the genes of the MALT lymphoma antibodies and the possible role antigenic selection within the tumor.
pubmed:language
ger
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0070-4113
pubmed:author
pubmed:issnType
Print
pubmed:volume
80
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
308-11
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
[Hybridomas as tools for research on MALT type lymphomas].
pubmed:affiliation
Pathologisches Institut, Universität Würzburg.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't