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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1997-5-15
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pubmed:abstractText |
The effect of recombinant porcine growth hormone (pGH) treatment on pituitary function was evaluated in young pigs. Piglets received intraperitoneal recombinant pGH implants (0.5 mg/d sustained release) or vehicle implants beginning at 3 d of age. Ten piglets were sacrificed at 4 and 6 wk of age (five piglets/treatment group) for the collection of pituitary glands, blood, and liver tissue. Blood samples also were drawn at 3 and 12 d of age. Serum concentrations of GH, prolactin (PRL), thyroid-stimulating hormone (TSH), insulin-like growth factor-1 (IGF-1) and IGF-2 were evaluated. Levels of IGF-1 and IGF-2 mRNA were determined in liver samples. Treatment with GH increased circulating levels of GH and IGF-1 (P < 0.01), but not PRL, TSH, or IGF-2. Hepatic IGF-1, but not IGF-2, mRNA levels were increased by pGH (P < 0.001). Cultured pituitary cells from each animal were challenged with 0.1, 1, and 10 nM GH-releasing hormone (GHRH); 2 nM 8-Br-cAMP; or 100 nM phorbol myristate acetate. The release of GH from cultured pituitary cells was stimulated by all secretagogues (P < 0.001). The secretion of GH, but not PRL or TSH, in culture was inhibited by previous in vivo GH treatment (P < 0.001). Similarly, cellular GH, but not PRL or TSH, content was lower in the GH-implant group (P = 0.005). Cell cultures from 6-wk-old piglets secreted more GH, but not PRL or TSH, than cultures from 4-wk-old piglets (P < 0.05). Likewise, cellular GH, but not PRL or TSH, content was greatest in cultures from 6-wk-old animals (P = 0.002). Piglet growth was not affected by exogenous GH treatment (P = 0.67). These results demonstrate that exogenous pGH treatment selectively down-regulates somatotroph function in young pigs.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/8-Bromo Cyclic Adenosine...,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Hormone-Releasing Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor I,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor II,
http://linkedlifedata.com/resource/pubmed/chemical/Prolactin,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/Thyrotropin
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0739-7240
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
109-18
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9063653-8-Bromo Cyclic Adenosine Monophosphate,
pubmed-meshheading:9063653-Animals,
pubmed-meshheading:9063653-Animals, Newborn,
pubmed-meshheading:9063653-Cells, Cultured,
pubmed-meshheading:9063653-Growth Hormone,
pubmed-meshheading:9063653-Growth Hormone-Releasing Hormone,
pubmed-meshheading:9063653-Insulin-Like Growth Factor I,
pubmed-meshheading:9063653-Insulin-Like Growth Factor II,
pubmed-meshheading:9063653-Liver,
pubmed-meshheading:9063653-Pituitary Gland,
pubmed-meshheading:9063653-Prolactin,
pubmed-meshheading:9063653-RNA, Messenger,
pubmed-meshheading:9063653-Recombinant Proteins,
pubmed-meshheading:9063653-Swine,
pubmed-meshheading:9063653-Tetradecanoylphorbol Acetate,
pubmed-meshheading:9063653-Thyrotropin
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pubmed:year |
1997
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pubmed:articleTitle |
Suppression of somatotroph function induced by growth hormone treatment in neonatal pigs.
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pubmed:affiliation |
Animal Physiology Research Unit, United States Department of Agriculture, University of Missouri, Columbia 65211, USA.
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pubmed:publicationType |
Journal Article
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