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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1997-4-2
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pubmed:abstractText |
Monoclonal antibodies were raised against prostate-specific antigen (PSA) by immunization with purified free PSA, i.e. not in complex with any protease inhibitor (F-PSA) and PSA in complex with alpha1-anti-chymotrypsin (PSA-ACT). Epitope mapping of PSA using the established monoclonal antibody revealed a complex pattern of independent and partly overlapping antigenic domains in the PSA molecule. Four independent antigenic domains and at least three partly overlapping domains were exposed both in F-PSA and in the PSA-ACT complex, while one antigenic domain was specific for F-PSA. The different domains contained both continuous and discontinuous epitopes. The combination of antibodies recognizing antigenic domains exposed both in F-PSA and PSA-ACT made it possible to develop several highly sensitive sandwich immunoassays for determination of total PSA, i.e. F-PSA + PSA-ACT, with the same molar response for F-PSA and PSA-ACT. Assays specific for F-PSA (cross-reactivity between F-PSA and PSA-ACT < 1%) were developed by the combination of antibodies recognizing epitopes exposed only in F-PSA and antibodies recognizing epitopes exposed both in F-PSA and PSA-ACT.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-1376193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-1700161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-1702714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-1707989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-1716536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-1724606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-2422647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-2436946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-2462065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-2466464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-2472789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-2824146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-3611349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-3691800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-3902893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-6375455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-6840874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-7504592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-7505968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-7516829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-7521782,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-7523709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-7544482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-7544581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-7586521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-7685416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-7691437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-7691441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-7691442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-7694784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9062397-89106
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0007-0920
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
75
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
789-97
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9062397-Animals,
pubmed-meshheading:9062397-Antibodies, Monoclonal,
pubmed-meshheading:9062397-Blotting, Western,
pubmed-meshheading:9062397-Cross Reactions,
pubmed-meshheading:9062397-Dose-Response Relationship, Immunologic,
pubmed-meshheading:9062397-Epitopes,
pubmed-meshheading:9062397-Female,
pubmed-meshheading:9062397-Humans,
pubmed-meshheading:9062397-Immunoenzyme Techniques,
pubmed-meshheading:9062397-Male,
pubmed-meshheading:9062397-Mice,
pubmed-meshheading:9062397-Mice, Inbred BALB C,
pubmed-meshheading:9062397-Prostate-Specific Antigen,
pubmed-meshheading:9062397-alpha-Macroglobulins
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pubmed:year |
1997
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pubmed:articleTitle |
Antigenic determinants of prostate-specific antigen (PSA) and development of assays specific for different forms of PSA.
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pubmed:affiliation |
CanAg Diagnostics AB, Gothenburg, Sweden.
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pubmed:publicationType |
Journal Article
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