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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1997-4-11
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pubmed:databankReference |
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pubmed:abstractText |
The human endogenous retrovirus type K (HERV-K) family codes for the human teratocarcinoma-derived retrovirus (HTDV) particles. The existence of the envelope protein (ENV) of HERV-K encoded by the subgenomic env mRNA has not yet been demonstrated. To study the genetic requirements for successful expression of ENV, we have constructed a series of recombinant HERV-K env expression vectors for infection and transfection experiments in insect cells and mammalian cells, respectively. Six baculovirus constructs bearing full-length or truncated HERV-K env with or without homologous or heterologous signal peptides were used for infections of insect cells. All recombinant baculoviruses yielded ENV proteins with the expected molecular masses. The full-length 80- to 90-kDa HERV-K ENV protein including the cORF leader sequence was glycosylated in insect cells. In addition, the 14-kDa cORF protein was expressed due to splicing of the full-length env mRNA. The ENV precursor protein is not cleaved to the surface (SU) and transmembrane (TM) glycoproteins; it does not appear on the surface of infected insect cells and is not secreted into the medium. For ENV expression in COS cells, plasmid vectors harboring the cytomegalovirus immediate-early promoter/intron A element and the tissue plasminogen activator (t-PA) signal peptide or the homologous HERV-K signal peptide upstream of the env gene were employed. Glycosylated and uncleaved ENV was expressed as in GH teratocarcinoma cells but at higher levels. The heterologous t-PA signal sequence was instrumental for expression of HERV-K ENV on the cell surface. Hence, we have shown for the first time that the HERV-K env gene has the potential to be expressed as a full-length envelope protein with appropriate glycosylation. In addition, our data provide explanations for the lack of infectivity of HERV-K/HTDV particles.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-11894899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-1310184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-1318614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-1650459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-1690394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-1690852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-1846947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-189071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-1955461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-2076345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-2494430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-2684548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-2744488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-3021993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-3149521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-3477816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-3714490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-3896128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-4032478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-6188345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-6202829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-6310154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-6423828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-7063411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-7301588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-7635468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-7645262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-7888184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-7924431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-7983704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-8289380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-8356806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-8372456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-8421897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-8429302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-8506289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-8512750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-8643549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-8648735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9060628-8797733
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
71
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2747-56
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9060628-Animals,
pubmed-meshheading:9060628-Antibodies, Viral,
pubmed-meshheading:9060628-Base Sequence,
pubmed-meshheading:9060628-COS Cells,
pubmed-meshheading:9060628-Cloning, Molecular,
pubmed-meshheading:9060628-DNA, Viral,
pubmed-meshheading:9060628-Gene Expression,
pubmed-meshheading:9060628-Gene Products, env,
pubmed-meshheading:9060628-Glycosylation,
pubmed-meshheading:9060628-Humans,
pubmed-meshheading:9060628-Molecular Sequence Data,
pubmed-meshheading:9060628-Rabbits,
pubmed-meshheading:9060628-Retroviridae,
pubmed-meshheading:9060628-Spodoptera,
pubmed-meshheading:9060628-Tumor Cells, Cultured
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pubmed:year |
1997
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pubmed:articleTitle |
Expression of human endogenous retrovirus type K envelope glycoprotein in insect and mammalian cells.
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pubmed:affiliation |
Paul-Ehrlich-Institut, Langen, Germany. Toenjes@em.uni-frankfurt.de
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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