Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1997-4-3
|
| pubmed:abstractText |
We previously demonstrated that proliferation of terminally differentiated Th1 clones depends primarily on an interleukin-12 (IL-12)-paracrine mechanism mediated by their interactions with antigen-presenting cells (APC) rather than on an IL-2-autocrine mechanism. Such a Th1 clone (4-86, C57BL/6 origin) was cultured with recombinant IL-12 (rIL-12) in the absence of either antigen or APC. Some cells survived for several passages of culture with only rIL-12, and by limiting dilution, several clones highly reactive to rIL-12 alone were obtained. One of these clones, designated 2D6, was found to proliferate strongly in response to less than 1 pg/mL of rIL-12. This clone exhibited the following surface phenotypes: CD3+, T cell receptor (TCR) alpha beta+, Vbeta11+, NK-1.1-; CD4-CD8-; LFA-1+, ICAM-1+; and CD28+, CD80+, CD86+, CTLA-4-. In accordance with high responsiveness to IL-12, 2D6 cells were also found to express IL-12 receptor (IL-12R) as detected by incubation with rIL-12 and then staining with anti-IL-12 monoclonal antibody (mAb). Stimulation of 2D6 with rIL-12 resulted in the expression of interferon-gamma (IFN-gamma) and IL-10 mRNAs and production of these cytokines. The 2D6 clone responded to IL-2 (vigorously), IL-7 (moderately), and IL-4 (mildly) in addition to IL-12. However, the Ab capture assay using anti-IL-12 mAb enabled us to quantify IL-12-specific activity contained in a given sample. Thus, this study describes the unique features of the IL-12-responsive T cell clone and demonstrates the utilization of this clone in the quantitation of a specific IL-12 activity.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0741-5400
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
61
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
346-52
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9060458-Animals,
pubmed-meshheading:9060458-Biological Assay,
pubmed-meshheading:9060458-Cell Aggregation,
pubmed-meshheading:9060458-Cell Division,
pubmed-meshheading:9060458-Cell Line,
pubmed-meshheading:9060458-Cytokines,
pubmed-meshheading:9060458-Interleukin-12,
pubmed-meshheading:9060458-Mice,
pubmed-meshheading:9060458-Mice, Inbred C57BL,
pubmed-meshheading:9060458-Phenotype,
pubmed-meshheading:9060458-Receptors, Interleukin,
pubmed-meshheading:9060458-Receptors, Interleukin-12,
pubmed-meshheading:9060458-Th1 Cells
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Establishment of an IL-12-responsive T cell clone: its characterization and utilization in the quantitation of IL-12 activity.
|
| pubmed:affiliation |
Biomedical Research Center, Osaka University Medical School, Suita, Japan.
|
| pubmed:publicationType |
Journal Article
|