Linked Life Data

9056674

Source:http://linkedlifedata.com/resource/pubmed/id/9056674

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1997-6-27
pubmed:abstractText
AML1 may play a role in growth and differentiation of cells along erythroid and/or megakaryocytic lineages, because a significant level of the AML1 gene is expressed in these cells. We overexpressed AML1a (without the transcription-activating domain) and AML1b (with the domain) proteins in K562 leukemia cells, which can be induced to differentiate into hemoglobin-producing cells and megakaryocytes. The AML1a-transfected K562 cells had a reduced capacity to differentiate in the presence of sodium n-butyrate but not in the presence of other inducers, such as hemin, 1-beta-D-arabinofuranosylcytosine, and herbimycin A. The AML1 antisense oligodeoxynucleotide but not the sense oligomer recovered its differentiation-inducing capacity in the presence of butyrate. On the other hand, AML1b conferred a similar differentiation-inducing capacity upon K562 cells transfected with vector alone. AML1a expression was associated with enhanced sensitivity to megakaryocytic differentiation induced by phorbol ester. These results provide evidence that AML1 proteins play a role in erythroid and megakaryocytic differentiation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1044-9523
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
319-26
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:9056674-Antigens, CD, pubmed-meshheading:9056674-Butyric Acid, pubmed-meshheading:9056674-Butyric Acids, pubmed-meshheading:9056674-Cell Differentiation, pubmed-meshheading:9056674-Core Binding Factor Alpha 2 Subunit, pubmed-meshheading:9056674-DNA-Binding Proteins, pubmed-meshheading:9056674-Erythroblasts, pubmed-meshheading:9056674-Gene Expression Regulation, Neoplastic, pubmed-meshheading:9056674-Glycophorin, pubmed-meshheading:9056674-Humans, pubmed-meshheading:9056674-Leukemia, Myelogenous, Chronic, BCR-ABL Positive, pubmed-meshheading:9056674-Megakaryocytes, pubmed-meshheading:9056674-Proto-Oncogene Proteins, pubmed-meshheading:9056674-RNA, Messenger, pubmed-meshheading:9056674-Tetradecanoylphorbol Acetate, pubmed-meshheading:9056674-Transcription Factors, pubmed-meshheading:9056674-Tumor Cells, Cultured
pubmed:year
1997
pubmed:articleTitle
AML1a but not AML1b inhibits erythroid differentiation induced by sodium butyrate and enhances the megakaryocytic differentiation of K562 leukemia cells.
pubmed:affiliation
Department of Chemotherapy, Saitama Cancer Center Research Institute, Ina, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't