Linked Life Data

9056241

Source:http://linkedlifedata.com/resource/pubmed/id/9056241

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-4-16
pubmed:databankReference
pubmed:abstractText
The genes possibly encoding the b subunit (50 kDa) of the Cl(-)-translocating ATPase of Acetabularia acetabulum were cloned from total RNA and from poly(A)+ RNA and sequenced. The deduced amino acid sequence of the open reading frame consisted of 478 amino acids and showed high similarity to the beta subunit of chloroplast F1-ATPase. Gene fragments encoding the putative beta subunit of chloroplast F1- (273 bp) and mitochondrial F1-ATPases (332 bp) were also cloned from A. acetabulum and sequenced, respectively. The deduced amino acid sequence of the chloroplast F1-ATPase showed 92.5% identity to be primary structure of the b subunit of the Cl(-)-translocating ATPase, while the nucleotide sequences were 79.9% identical. The deduced amino acid sequence of the latter was 77.3% identical to that of the b subunit of the Cl(-)-translocating ATPase and the nucleotide sequences were 67.5% identical. By Northern analysis, these three beta-like genes were demonstrated to be transcribed with different sizes of RNA species. A putative chloroplast F1-beta fragment also hybridized with chloroplast DNA isolated from the organism.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0003-9861
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
339
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
115-24
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9056241-Acetabularia, pubmed-meshheading:9056241-Adenosine Triphosphatases, pubmed-meshheading:9056241-Amino Acid Sequence, pubmed-meshheading:9056241-Animals, pubmed-meshheading:9056241-Anion Transport Proteins, pubmed-meshheading:9056241-Bacterial Proteins, pubmed-meshheading:9056241-Base Sequence, pubmed-meshheading:9056241-Biological Transport, Active, pubmed-meshheading:9056241-Genes, Plant, pubmed-meshheading:9056241-Molecular Sequence Data, pubmed-meshheading:9056241-Plant Proteins, pubmed-meshheading:9056241-RNA, Messenger, pubmed-meshheading:9056241-Rats, pubmed-meshheading:9056241-Sequence Alignment, pubmed-meshheading:9056241-Sequence Homology, Amino Acid, pubmed-meshheading:9056241-Sequence Homology, Nucleic Acid, pubmed-meshheading:9056241-Species Specificity
pubmed:year
1997
pubmed:articleTitle
The primary structure of the Cl(-)-translocating ATPase, b subunit of Acetabularia acetabulum, which belongs to the F-type ATPase family.
pubmed:affiliation
Faculty of Health and Welfare Science, Okayama Prefectural University, Soja, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't