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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
|
| pubmed:dateCreated |
1997-4-17
|
| pubmed:abstractText |
A pair of oligomers of 20 and 23 bp were designed for amplifying a 381 bp sequence from glycoprotein IV gene of bovine herpesvirus 1. The primer pairs were used for amplifying genomic DNA of BHV-1 directly from cell culture fluids under different experimental conditions such as, untreated cell culture fluid, thermal denaturation and proteinase K treatment in presence of detergent. The results reveal that direct thermal denaturation of cell culture fluid is sufficient to detect the virus by polymerase chain reaction.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0019-5189
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
34
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1169-71
|
| pubmed:dateRevised |
2004-2-9
|
| pubmed:meshHeading |
pubmed-meshheading:9055637-Animals,
pubmed-meshheading:9055637-Base Sequence,
pubmed-meshheading:9055637-Cattle,
pubmed-meshheading:9055637-Cattle Diseases,
pubmed-meshheading:9055637-DNA, Viral,
pubmed-meshheading:9055637-DNA Primers,
pubmed-meshheading:9055637-Herpesviridae Infections,
pubmed-meshheading:9055637-Herpesvirus 1, Bovine,
pubmed-meshheading:9055637-Polymerase Chain Reaction,
pubmed-meshheading:9055637-Viral Proteins
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Direct detection of bovine herpes virus-1 DNA from cell culture fluids using polymerase chain reaction.
|
| pubmed:affiliation |
Indian Veterinary Research Institute, Bangalore, India.
|
| pubmed:publicationType |
Journal Article
|