Linked Life Data

9054396

Source:http://linkedlifedata.com/resource/pubmed/id/9054396

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1997-4-17
pubmed:abstractText
Phospholipase D (PLD; EC 3.1.4.4) has been proposed to be involved in a number of cellular processes including transmembrane signaling and membrane deterioration. PLD previously described from various plant sources generally requires millimolar ranges of calcium for optimal activity. In this study, we genetically suppressed the expression of this conventional PLD in Arabidopsis by introducing an antisense PLD cDNA. However, both the antisense transgenic and wild-type plants showed comparable PLD activity in the presence of submicromolar concentrations of calcium and phosphatidylinositol 4, 5-bisphosphate using phosphatidylcholine as a substrate. This new PLD activity was partially stimulated by phosphatidylinositol 4-phosphate, but not by other phospholipids, including phosphatidylinositol, phosphatidylserine, phosphatidylglycerol, phosphatidic acid, or phosphatidylcholine. Its requirement for polyphosphoinositides was further supported by its ability to be inhibited by neomycin. The polyphosphoinositide-dependent PLD requires calcium for activity, but no magnesium. The calcium stimulation was observed in the nanomolar range and reached a plateau at 5 microM calcium. The findings of this study demonstrate the presence of different PLDs that are regulated in a distinct manner in plants. The potential significance of a PLD that is regulated by polyphosphoinositides and physiological concentrations of Ca2+ is discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
272
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7048-54
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Identification and characterization of a novel plant phospholipase D that requires polyphosphoinositides and submicromolar calcium for activity in Arabidopsis.
pubmed:affiliation
Department of Biochemistry, Kansas State University, Manhattan, Kansas 66506, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.