Linked Life Data

9054124

Source:http://linkedlifedata.com/resource/pubmed/id/9054124

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3-4
pubmed:dateCreated
1997-3-11
pubmed:abstractText
The Gram-positive transposon Tn916 was introduced into two strains of Haemophilus paragallinarum by electroporation of the suicide plasmid pAM120 (pGL101::Tn916). Tetracycline resistant mutants of H. paragallinarum strains Tw-1 and 221 were recovered at frequencies of 2.1 x 10(-6) and 4.5 x 10(-7) per viable cell electroporated, respectively. Tn916 generates stable single insertions within different sites of the H. paragallinarum chromosome. One Tw-1::Tn916 mutant had the Tn916 insertion in the cryptic plasmid p250. This study indicates the potential use of Tn916 as a genetic tool for insertional mutagenesis of H. paragallinarum.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0378-1135
pubmed:author
pubmed:issnType
Print
pubmed:volume
48
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
283-91
pubmed:dateRevised
2006-5-1
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Transposon mutagenesis of Haemophilus paragallinarum with Tn916.
pubmed:affiliation
Department of Avian Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602-487, USA.
pubmed:publicationType
Journal Article