Linked Life Data

9049064

Source:http://linkedlifedata.com/resource/pubmed/id/9049064

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-6-2
pubmed:abstractText
The presence and the possibility of induction of argininosuccinate synthetase in a glial cell line were investigated. For this purpose, antisera were produced against peptides representing partial sequences 196-222 and 337-349, respectively, of the mouse liver enzyme. Both antisera were shown to be monospecific for argininosuccinate synthetase. In Western blot experiments, immunoreactivity was found in mouse liver and brain homogenates. Only weak immunoreactivity was detectable in homogenates of cultured glioma cells, C6-BU-1. However, when the glioma cells were treated with either bacterial lipopolysaccharide, interferon-gamma, or a combination of both, argininosuccinate synthetase immunoreactivity was increased. The findings demonstrate that this enzyme is present in glial cells and is induced under conditions which stimulate persistent production of nitric oxide. The antisera will be a valuable tool for further investigations on arginine synthesis in brain as well as peripheral cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1431-6730
pubmed:author
pubmed:issnType
Print
pubmed:volume
378
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
47-50
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Presence of argininosuccinate synthetase in glial cells as revealed by peptide-specific antisera.
pubmed:affiliation
Physiologisch-chemisches Institut der Universität, Tübingen, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't