Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1997-3-20
pubmed:abstractText
Vascular endothelial cells are constantly in contact with oxyradicals and must be especially well equipped to resist their toxic effects and generate appropriate physiological responses. Despite the importance of oxyradicals in the physiopathology of the vascular endothelium, the mechanisms regulating the oxidative response of endothelial cells are poorly understood. In the present study, we observed that H2O2 in concentrations that induced severe fragmentation of F-actin in fibroblasts rather induced a reorganization of F-actin in primary cultures of human umbilical vein endothelial cells (HUVECs) that was characterized by the accumulation of stress fibers, the recruitment of vinculin to focal adhesions, and the loss of membrane ruffles, H2O2 also induced in these cells a strong (10- to 14-fold) activation of the p38 mitogen-activated protein (MAP) kinase, which resulted in activation of MAP kinase-activated protein kinase-2/3 and phosphorylation of the F-actin polymerization modulator, heat shock protein 27 (HSP27). The MAP kinases extracellular-regulated kinase, and c-Jun N-terminal kinase/stress-activated protein kinase were only slightly increased by these treatments. Inhibiting p38 activity with the highly specific inhibitor SB203580 blocked the H2O2-induced endothelial microfilament responses. Moreover, fibroblasts acquired an endothelium-like SB203580-sensitive actin response when HSP27 concentration was increased by gene transfection to the same high level as found in HUVECs. The results indicate that activation of p38 MAP kinase in cells such as endothelial cells, which naturally express high level of HSP27, plays a central role in modulating microfilament responses to oxidative stress. Consequently, the p38 MAP kinase pathway may participate in the several oxyradical-activated functions of the endothelium that are associated with reorganization of microfilament network.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Actins, http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Calmodulin-Dependent..., http://linkedlifedata.com/resource/pubmed/chemical/Heat-Shock Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Hydrogen Peroxide, http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides..., http://linkedlifedata.com/resource/pubmed/chemical/JNK Mitogen-Activated Protein..., http://linkedlifedata.com/resource/pubmed/chemical/MAP-kinase-activated kinase 2, http://linkedlifedata.com/resource/pubmed/chemical/MAP-kinase-activated kinase 3, http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 1, http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Vitamin K, http://linkedlifedata.com/resource/pubmed/chemical/p38 Mitogen-Activated Protein...
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0009-7330
pubmed:author
pubmed:issnType
Print
pubmed:volume
80
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
383-92
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:9048659-Actins, pubmed-meshheading:9048659-Animals, pubmed-meshheading:9048659-CHO Cells, pubmed-meshheading:9048659-Calcium-Calmodulin-Dependent Protein Kinases, pubmed-meshheading:9048659-Cattle, pubmed-meshheading:9048659-Cells, Cultured, pubmed-meshheading:9048659-Cricetinae, pubmed-meshheading:9048659-Dose-Response Relationship, Drug, pubmed-meshheading:9048659-Endothelium, Vascular, pubmed-meshheading:9048659-Fluorescent Antibody Technique, Direct, pubmed-meshheading:9048659-Heat-Shock Proteins, pubmed-meshheading:9048659-Humans, pubmed-meshheading:9048659-Hydrogen Peroxide, pubmed-meshheading:9048659-Intracellular Signaling Peptides and Proteins, pubmed-meshheading:9048659-JNK Mitogen-Activated Protein Kinases, pubmed-meshheading:9048659-Mitogen-Activated Protein Kinase 1, pubmed-meshheading:9048659-Mitogen-Activated Protein Kinases, pubmed-meshheading:9048659-Oxidative Stress, pubmed-meshheading:9048659-Protein-Serine-Threonine Kinases, pubmed-meshheading:9048659-Pulmonary Artery, pubmed-meshheading:9048659-Umbilical Veins, pubmed-meshheading:9048659-Vitamin K, pubmed-meshheading:9048659-p38 Mitogen-Activated Protein Kinases
pubmed:year
1997
pubmed:articleTitle
Oxidative stress-induced actin reorganization mediated by the p38 mitogen-activated protein kinase/heat shock protein 27 pathway in vascular endothelial cells.
pubmed:affiliation
Centre de recherche en cancérologie de l'Université Laval, L'Hôtel-Dieu de Québec, Canada. Jacques.Huot@phc.ulaval.ca
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't