Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1997-8-4
pubmed:abstractText
Protein 4.1, originally identified as a component of the membrane-skeleton of the red blood cell, has also been localized in the nucleus of mammalian cells. To learn more about nuclear 4.1 protein, we have analyzed the nature of its association with the nuclear structure in comparison with SC35 and snRNP antigens, splicing proteins of the nuclear speckle domains. When MDCK or HeLa cells were digested with DNase I and washed in the presence of high salt (2 M NaCl), snRNP antigens were extracted whereas protein 4.1 and SC35 remained colocalizing in nuclear speckles. In cells treated with RNase A or heat shocked, nuclear 4.1 distribution also resembled that of SC35. Experiments carried out in transcriptionally active nuclei showed that protein 4.1 distributed in irregularly shaped speckles which appeared to be interconnected. During transcriptional inhibition, protein 4.1 accumulated in rounded speckles lacking interconnections. When cells were released from transcriptional inhibition, protein 4.1 redistributed back to the interconnected speckle pattern of transcriptionally active cells, as it was also observed for SC35. Finally, coprecipitation of 4.1 and SC35 proteins from RNase A digested HeLa nuclei further indicates that these two proteins are associated, forming part of the nuclear speckle domains to which they attach more tightly than snRNP antigens.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Cytoskeletal Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonuclease I, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Neuropeptides, http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Ribonuclease, Pancreatic, http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleoproteins, Small Nuclear, http://linkedlifedata.com/resource/pubmed/chemical/SRSF2 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/erythrocyte membrane band 4.1..., http://linkedlifedata.com/resource/pubmed/chemical/erythrocyte membrane protein band...
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9533
pubmed:author
pubmed:issnType
Print
pubmed:volume
110 ( Pt 2)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
239-47
pubmed:dateRevised
2011-9-27
pubmed:meshHeading
pubmed-meshheading:9044054-Animals, pubmed-meshheading:9044054-Binding Sites, pubmed-meshheading:9044054-Cell Line, pubmed-meshheading:9044054-Cell Nucleus, pubmed-meshheading:9044054-Cytoskeletal Proteins, pubmed-meshheading:9044054-Deoxyribonuclease I, pubmed-meshheading:9044054-Dogs, pubmed-meshheading:9044054-Fluorescent Antibody Technique, Indirect, pubmed-meshheading:9044054-HeLa Cells, pubmed-meshheading:9044054-Heat-Shock Response, pubmed-meshheading:9044054-Humans, pubmed-meshheading:9044054-Membrane Proteins, pubmed-meshheading:9044054-Neuropeptides, pubmed-meshheading:9044054-Nuclear Proteins, pubmed-meshheading:9044054-Precipitin Tests, pubmed-meshheading:9044054-Ribonuclease, Pancreatic, pubmed-meshheading:9044054-Ribonucleoproteins, pubmed-meshheading:9044054-Ribonucleoproteins, Small Nuclear, pubmed-meshheading:9044054-Temperature, pubmed-meshheading:9044054-Transcription, Genetic
pubmed:year
1997
pubmed:articleTitle
Transcription-dependent redistribution of nuclear protein 4.1 to SC35-enriched nuclear domains.
pubmed:affiliation
Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma de Madrid, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't