Linked Life Data

9043645

Source:http://linkedlifedata.com/resource/pubmed/id/9043645

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-6-13
pubmed:abstractText
In the present report we demonstrate the in vitro effects of free radicals on an ecto-ATP diphosphohydrolase activity (apyrase, EC 3.6.1.5) from rat blood platelets. Rat blood platelets were exposed to an oxidant-generating system (H2O2/Fe2+/ascorbate) and the ATP diphosphohydrolase activity was inhibited. The enzyme inhibition was prevented by glutathione (GSH) and cysteine but not by trolox as a vitamin E analogue. The TBARS (thiobarbituric acid reactive substances) assay and the determination of sulphydryl groups indicate that the inhibition of the enzyme activity in resting platelets is not related to lipid peroxidation or to oxidation of sulphydryl residues. These results demonstrate the susceptibility of ATP diphosphohydrolase activity from platelets to free radicals and suggest that amino acid residues which are essential for the enzyme function are probably modified.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1039-9712
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
161-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Free radical-induced inhibition of ATP diphosphohydrolase activity (EC 3.6.1.5) from rat blood platelets.
pubmed:affiliation
Universidade Federal do Rio Grande do Sul, Departamento de Bioquimica Rua Sarmento Leite, Porto Alegre, RS, Brasil.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't