Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1997-3-13
|
| pubmed:abstractText |
These studies were undertaken to assess the estrogenic and antiestrogenic effects of dietary genistein. To determine estrogenic effects, genistein was mixed into a modified AIN-76 or AIN-93G semipurified diet at 0 (negative control), 150, 375 or 750 microg/g and 17, beta-estradiol at 1.0 microg/g and fed to ovariectomized 70-d-old Sprague-Dawley rats. Estrogenic potency was determined by analyzing uterine weight, mammary gland development, plasma prolactin and expression of uterine c-fos. Dietary genistein (375 and 750 microg/g) increased uterine wet and dry weights (P < 0.05). Mammary gland regression following ovariectomy was significantly inhibited by dietary genistein at 750 microg/g (P < 0.05). Plasma prolactin was significantly greater in ovariectomized rats fed genistein (750 microg/g) compared with comparable rats not receiving genistein. The relative binding affinity of genistein to the estrogen receptor (ER) was 0.01 that of estradiol. Genistein (750 microg/g) induced the uterine expression of c-fos. To evaluate potential antiestrogenic effects, genistein and estradiol were mixed into the modified AIN diets at the doses noted above and fed to ovariectomized rats. Dietary genistein (375 or 750 microg/g) did not inhibit the effects of estradiol on uterine weight, mammary gland development or plasma prolactin. Serum concentration of total genistein (conjugated plus free) in rats fed 750 microg/g was 2.2 micromol/L and free genistein was 0.4 micromol/L. Administration of dietary genistein at 750 microg/g can exert estrogenic effects in the uterus, mammary gland and hypothalamic/pituitary axis. Dietary genistein (750 microg/g) did not antagonize the action of estradiol in estradiol-supplemented ovariectomized rats or in intact rats.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Estradiol,
http://linkedlifedata.com/resource/pubmed/chemical/Estrogens, Non-Steroidal,
http://linkedlifedata.com/resource/pubmed/chemical/Genistein,
http://linkedlifedata.com/resource/pubmed/chemical/Isoflavones,
http://linkedlifedata.com/resource/pubmed/chemical/Prolactin,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fos,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Estrogen
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0022-3166
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
127
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
263-9
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:9039826-Administration, Oral,
pubmed-meshheading:9039826-Animal Feed,
pubmed-meshheading:9039826-Animals,
pubmed-meshheading:9039826-Binding, Competitive,
pubmed-meshheading:9039826-Dose-Response Relationship, Drug,
pubmed-meshheading:9039826-Estradiol,
pubmed-meshheading:9039826-Estrogens, Non-Steroidal,
pubmed-meshheading:9039826-Female,
pubmed-meshheading:9039826-Genistein,
pubmed-meshheading:9039826-Hypothalamo-Hypophyseal System,
pubmed-meshheading:9039826-Isoflavones,
pubmed-meshheading:9039826-Mammary Glands, Animal,
pubmed-meshheading:9039826-Organ Size,
pubmed-meshheading:9039826-Ovariectomy,
pubmed-meshheading:9039826-Prolactin,
pubmed-meshheading:9039826-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:9039826-Rats,
pubmed-meshheading:9039826-Rats, Sprague-Dawley,
pubmed-meshheading:9039826-Receptors, Estrogen,
pubmed-meshheading:9039826-Uterus
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Dietary genistein exerts estrogenic effects upon the uterus, mammary gland and the hypothalamic/pituitary axis in rats.
|
| pubmed:affiliation |
Department of Food Science and Human Nutrition, Michigan State University, E. Lansing 48824, USA.
|
| pubmed:publicationType |
Journal Article
|