Linked Life Data

9038931

Source:http://linkedlifedata.com/resource/pubmed/id/9038931

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1 Pt 2
pubmed:dateCreated
1997-3-31
pubmed:abstractText
We have characterized the interaction of endothelin (ET) with cultured neonatal rat ventricular myocytes. Binding studies indicate a single population of ETA receptors [53,000 sites/cell, apparent dissociation constant (Kd) for ET-1 approximately 0.07 nM]. Analysis of mRNA levels for ET receptors using 35 cycles of reverse transcriptase-polymerase chain reaction demonstrates the presence of only ETA-receptor message. Studies with ET-1 and a variety of congeners and antagonists indicate that ETA receptors couple to both the stimulation of phosphoinositide turnover and the inhibition of adenylyl cyclase. In myocytes transfected with an atrial natriuretic factor (ANF) promoter linked to a luciferase reporter gene, ET-1 stimulates luciferase expression through an ETA receptor. These data indicate that the ETA receptor is the exclusive receptor on neonatal ventricular myocytes and that this receptor couples to both phosphoinositide hydrolysis and adenylyl cyclase. ET-1 also induces a threefold increase in mitogen-activated protein kinase (MAPK) activity, an effect that is not sensitive to pertussis toxin (PTx). By contrast, ET-stimulated ANF-luciferase expression is partially inhibited by treatment of cells with PTx, suggesting that both PTx-sensitive (Gi) and PTx-insensitive (Gq) pathways mediate the effects of ET-1 on ANF gene expression in neonatal myocytes and that hormonal regulation of ANF expression may utilize pathways in addition to the activation of MAPK.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
272
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
H130-7
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9038931-Animals, pubmed-meshheading:9038931-Atrial Natriuretic Factor, pubmed-meshheading:9038931-Calcium-Calmodulin-Dependent Protein Kinases, pubmed-meshheading:9038931-Cyclic AMP, pubmed-meshheading:9038931-Endothelins, pubmed-meshheading:9038931-GTP-Binding Proteins, pubmed-meshheading:9038931-Gene Expression, pubmed-meshheading:9038931-Genes, pubmed-meshheading:9038931-Ligands, pubmed-meshheading:9038931-Luciferases, pubmed-meshheading:9038931-Phosphatidylinositols, pubmed-meshheading:9038931-Polymerase Chain Reaction, pubmed-meshheading:9038931-Promoter Regions, Genetic, pubmed-meshheading:9038931-RNA, Messenger, pubmed-meshheading:9038931-Rats, pubmed-meshheading:9038931-Rats, Sprague-Dawley, pubmed-meshheading:9038931-Receptors, Endothelin, pubmed-meshheading:9038931-Signal Transduction
pubmed:year
1997
pubmed:articleTitle
Endothelin ETA receptor regulates signaling and ANF gene expression via multiple G protein-linked pathways.
pubmed:affiliation
Department of pharmacology, University of California, San Diego, La Jolla 92093, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't