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rdf:type |
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lifeskim:mentions |
umls-concept:C0011306,
umls-concept:C0086418,
umls-concept:C0243125,
umls-concept:C0243144,
umls-concept:C0304925,
umls-concept:C0439855,
umls-concept:C0699900,
umls-concept:C1167622,
umls-concept:C1533691,
umls-concept:C1832073,
umls-concept:C1880497,
umls-concept:C1996904
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pubmed:issue |
1
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pubmed:dateCreated |
1997-3-7
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pubmed:abstractText |
The handling of free and IgG-complexed dinitrophenylated human serum albumin (DNP-HSA) by human dendritic cells (DC) cultured with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) was studied. It has been shown that the amount of uncomplexed or IgG-complexed antigen required by DC to start an immune response is low compared with other antigen-presenting cells. We therefore examined whether such efficient presentation of immune complexes is due to an enhanced Fc gamma RII-mediated endocytosis or to a specialized and efficient antigen handling, i.e., macropinocytosis. The Fc gamma RII expression was found to be heterogeneous on the GM-CSF- and IL-4-cultured DC, i.e. it ranges from low to high expression. The handling of antigen and immune complexes revealed, that the level of binding and uptake of IgG-DNP-HSA complexes by in vitro expanded DC is low compared with free antigen. Uncomplexed DNP-HSA is probably handled either by endocytosis via receptors being more abundant and/or efficient than the Fc gamma RII or via non-receptor-mediated endocytosis. The binding and uptake of IgG-complexed DNP-HSA was blocked by anti-Fc gamma RII antibody, indicating the specificity of the interaction.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-1317711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-1357074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-1383322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-1910679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-2109013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-2124207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-2144968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-2145580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-2303788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-2400808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-2526849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-2529342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-2666563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-6348167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-6715404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-6882394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-7525461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-7538534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-7540472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-7612895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-7629501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-7636231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-7678623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-7790816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-7798627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-8006603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-8045704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-8145033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038724-8260848
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
90
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
138-46
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9038724-Antigen-Antibody Complex,
pubmed-meshheading:9038724-Binding, Competitive,
pubmed-meshheading:9038724-Cell Culture Techniques,
pubmed-meshheading:9038724-Dendritic Cells,
pubmed-meshheading:9038724-Dinitrophenols,
pubmed-meshheading:9038724-Endocytosis,
pubmed-meshheading:9038724-Flow Cytometry,
pubmed-meshheading:9038724-Humans,
pubmed-meshheading:9038724-Immunoglobulin G,
pubmed-meshheading:9038724-Immunophenotyping,
pubmed-meshheading:9038724-Kupffer Cells,
pubmed-meshheading:9038724-Receptors, IgG,
pubmed-meshheading:9038724-Serum Albumin,
pubmed-meshheading:9038724-Temperature
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pubmed:year |
1997
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pubmed:articleTitle |
Human dendritic cells handling of binding, uptake and degradation of free and IgG-immune complexed dinitrophenylated human serum albumin in vitro.
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pubmed:affiliation |
Department of Clinical Microbiology, Faculty of Health Sciences, Linköping University, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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