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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1997-3-7
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pubmed:abstractText |
Previous work demonstrated that human cytotoxic T cells activated by superantigens can lyse major histocompatibility complex (MHC) class II-positive target cells as well as MHC class II-negative tumour cells coated with conjugates of monoclonal antibodies and superantigens. In order to decrease MHC class II affinity, and therefore unwanted binding of the superantigen staphylococcal enterotoxin A (SEA) to MHC class II molecules, a point mutation was introduced into the SEA gene. This mutation (SEAD227A) resulted in an approximately 3-log reduction of affinity to human leucocyte antigen (HLA)-DR, but cytotoxicity mediated by this mutant superantigen towards antibody-labelled tumour cells is as efficient as cytotoxicity mediated by the native superantigen. We therefore compared the T-cell activating potency of native and mutated SEA. Our data show that SEAD227A is 4- to 5-log less effective than native SEA when activation of resting T cells is assayed in terms of blast formation, expression of cell surface activation markers and cytokine release. Furthermore, presenting either SEA or SEAD227A to MHC class II-negative mononuclear cells by MHC class II-negative tumour cells did not result in significant blast formation of T cells, up-regulation of CD25 or cytokine release. This suggests that lysis of MHC class II-negative tumour cells is efficiently induced by monoclonal antibody targeted superantigen, while activation of resting T cells requires additional co-stimulatory signals.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038715-1330329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038715-1878125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038715-1899481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038715-1924393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038715-2210803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038715-2514231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038715-3100030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038715-3259256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038715-3507693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038715-7505257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038715-7530598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038715-7542584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038715-7553685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038715-7568219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038715-7595227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038715-7628431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038715-7635573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038715-7678619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038715-8013959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038715-8120374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038715-8509223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9038715-8566049
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
90
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
74-80
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9038715-Cell Culture Techniques,
pubmed-meshheading:9038715-Cytokines,
pubmed-meshheading:9038715-Cytotoxicity, Immunologic,
pubmed-meshheading:9038715-Enterotoxins,
pubmed-meshheading:9038715-HLA-D Antigens,
pubmed-meshheading:9038715-Humans,
pubmed-meshheading:9038715-Lymphocyte Activation,
pubmed-meshheading:9038715-Point Mutation,
pubmed-meshheading:9038715-Superantigens,
pubmed-meshheading:9038715-T-Lymphocytes,
pubmed-meshheading:9038715-Tumor Cells, Cultured
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pubmed:year |
1997
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pubmed:articleTitle |
T-cell stimulation and cytokine release induced by staphylococcal enterotoxin A (SEA) and the SEAD227A mutant.
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pubmed:affiliation |
Children's University Hospital, Department of Oncology/Haematology, Tübingen, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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