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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1997-3-25
|
| pubmed:abstractText |
Polyoxyethylene (20) sorbitan mono-fatty acid esters strongly enhanced the inactivation of lipopolysaccharide (LPS) by steam-heat treatment at 121 degrees C, as assayed by using the Limulus amebocyte lysate (LAL) and the pyrogen test. In an aqueous solution containing 0.1% surfactant, the decrease of LPS (1 microgram/ml) from E. coli 055:B5 at 121 degrees C followed first-order kinetics. Based on the LAL assay, 0.1% surfactant was essential to achieve 3-log cycle reduction of LPS with concomitant loss of pyrogenicity by steam-heat treatment for 20 min at 121 degrees C. Steam-heat treatment for 20 min at 121 degrees C in the absence of surfactant was insufficient to achieve depyrogenation. Polyoxyethylene (9) lauryl ether and decaglycerin mono-laurate similarly enhanced depyrogenation by steam-heat treatment. The effects of all the surfactants were concentration-dependent for all of the six kinds of LPS examined.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
1079-7440
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
50
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
360-5
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:9038081-Feasibility Studies,
pubmed-meshheading:9038081-Fever,
pubmed-meshheading:9038081-Hot Temperature,
pubmed-meshheading:9038081-Humans,
pubmed-meshheading:9038081-Infusions, Parenteral,
pubmed-meshheading:9038081-Lipopolysaccharides,
pubmed-meshheading:9038081-Surface-Active Agents
|
| pubmed:articleTitle |
Enhancing effect of non-ionic surfactant on the inactivation of lipopolysaccharide by steam-heat treatment I.
|
| pubmed:affiliation |
Pharmaceutical Research Center, Shiseido Company, Yokohama, Japan.
|
| pubmed:publicationType |
Journal Article
|