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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1997-5-9
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pubmed:databankReference | |
pubmed:abstractText |
A putative ecdysteroid UDP-glucosyltransferase (egt) gene was identified in the single nucleocapsid nucleopolyhedrovirus of Buzura suppressaria (BusuNPV). This is the first egt gene to be characterized in a SNPV, suggesting that egt genes are prevalent in nucleopolyhedroviruses and possibly in all baculoviruses. The open reading frame (ORF) of the gene is 1539 nucleotides (nt) long, encoding a putative protein (EGT) of 513 amino acids (aa) with a M of 58922. The 5' noncoding region contains three possible TATA boxes. A polyadenylation signal, AATAAA, was found downstream of the translation stop codon. A putative signal peptide of 16 residues was present at the N-terminus of the EGT. The BusuNPV egt gene has a high degree of nucleotide and amino acid sequence homology to multiple nucleocapsid (M) NPV egt genes, the highest being to the Spodoptera exigua MNPV egt. A phylogenetic tree of eleven known EGTs was constructed using maximum parsimony analysis.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0168-1702
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
47
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
91-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9037741-Amino Acid Sequence,
pubmed-meshheading:9037741-Animals,
pubmed-meshheading:9037741-Base Sequence,
pubmed-meshheading:9037741-DNA, Viral,
pubmed-meshheading:9037741-Glucosyltransferases,
pubmed-meshheading:9037741-Molecular Sequence Data,
pubmed-meshheading:9037741-Moths,
pubmed-meshheading:9037741-Nucleopolyhedrovirus,
pubmed-meshheading:9037741-Phylogeny,
pubmed-meshheading:9037741-Sequence Homology, Amino Acid,
pubmed-meshheading:9037741-Viral Proteins
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pubmed:year |
1997
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pubmed:articleTitle |
Characterization of the ecdysteroid UDP-glucosyltransferase gene of a single nucleocapsid nucleopolyhedrovirus of Buzura suppressaria.
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pubmed:affiliation |
Department of Virology, Wageningen Agricultural University, Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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