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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1997-5-8
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pubmed:abstractText |
In order to evaluate the conditions for optimal expression and immunogenicity of varicella-zoster virus (VZV) proteins in a herpes simplex virus-1 (HSV-1) vector, we selected the VZV glycoprotein E (gE), encoded by ORF 68 and the VZV product of ORF 62, an immediate-early major tegument protein (IE62). Three HSV/VZV recombinants were generated: (1) VZV gE protein coding sequences along with the promoter region were inserted into the thymidine kinase (TK) gene of HSV-1 strain KOS; (2) VZV gE expressed from the HSV-1 ICP4 promoter was inserted into the glycoprotein C (gC) gene of HSV-1 strain F; and (3) VZV IE62 protein coding sequences under the control of the HSV-1 ICP4 promoter were inserted into the gC gene of HSV-1 strain F. Immunoblot analysis and immunoperoxidase staining of infected cell monolayers demonstrated vector expression of VZV proteins. Following intracranial inoculation in mice, both VZV gE-HSV (TK) and VZV IE62-HSV (gC) induced an IgG response against VZV gE or VZV IE62. When tested in cytotoxicity assays using T-lymphocytes from VZV immune human donors, the range of precursor frequencies for T-lymphocytes that recognized VZV gE or VZV IE62 was similar whether these proteins were expressed by HSV-1 or a vaccinia vector. These experiments demonstrate that HSV-1 is a competent vector for expression of these VZV proteins and support the feasibility of engineering a combined vaccine for these closely related alpha-herpesviruses.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acyclovir,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Antiviral Agents,
http://linkedlifedata.com/resource/pubmed/chemical/IE62 protein, Human herpesvirus 3,
http://linkedlifedata.com/resource/pubmed/chemical/Immediate-Early Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Envelope Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/glycoprotein gp1, varicella-zoster...
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0166-3542
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
33
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
187-200
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9037375-Acyclovir,
pubmed-meshheading:9037375-Animals,
pubmed-meshheading:9037375-Antigens, Viral,
pubmed-meshheading:9037375-Antiviral Agents,
pubmed-meshheading:9037375-Blotting, Southern,
pubmed-meshheading:9037375-Cercopithecus aethiops,
pubmed-meshheading:9037375-Cytotoxicity Tests, Immunologic,
pubmed-meshheading:9037375-Genetic Vectors,
pubmed-meshheading:9037375-Guinea Pigs,
pubmed-meshheading:9037375-Herpes Simplex,
pubmed-meshheading:9037375-Herpesvirus 1, Human,
pubmed-meshheading:9037375-Herpesvirus 3, Human,
pubmed-meshheading:9037375-Humans,
pubmed-meshheading:9037375-Immediate-Early Proteins,
pubmed-meshheading:9037375-Immunoblotting,
pubmed-meshheading:9037375-Mice,
pubmed-meshheading:9037375-Mice, Inbred BALB C,
pubmed-meshheading:9037375-Recombinant Fusion Proteins,
pubmed-meshheading:9037375-Recombination, Genetic,
pubmed-meshheading:9037375-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:9037375-Trans-Activators,
pubmed-meshheading:9037375-Vero Cells,
pubmed-meshheading:9037375-Viral Envelope Proteins
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pubmed:year |
1997
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pubmed:articleTitle |
The synthesis and immunogenicity of varicella-zoster virus glycoprotein E and immediate-early protein (IE62) expressed in recombinant herpes simplex virus-1.
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pubmed:affiliation |
Department of Pediatrics, Stanford University School of Medicine, CA 94305-5119, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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