Linked Life Data

9035371

Source:http://linkedlifedata.com/resource/pubmed/id/9035371

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1997-5-29
pubmed:databankReference
pubmed:abstractText
A polymerase chain reaction (PCR) targeted to the central portion of the bovine herpesvirus 1 (BHV1) genome, and overlapping the 3' untranslated end of the gI glycoprotein, was used to amplify BHV1 genomic sequences. PCR products generated from cell cultures infected with BHV1.1 were consistently smaller than the corresponding products from cells infected with BHV1.2. The nature of the sequence differences between these isolates within the target region was found to be a consequence of variable numbers of small GC rich repeats, particularly the sequence 5'-G(A/T)CC-3', present in the region downstream of the gI coding region. Based on these differences a modified PCR protocol which readily discriminated between several BHV1.1 and BHV1.2 strains was devised.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0920-8569
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
263-8
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Bovine herpesvirus 1 isolates contain variable copy numbers of GC-rich tandem repeats in the gI non-coding regions of their genomes.
pubmed:affiliation
Pathobiology Section, Agriculture and Agri-Food Canada, Ontario, Canada.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.