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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1997-5-22
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pubmed:abstractText |
Stimulation of rat mesangial cells with platelet-derived growth factor BB (PDGF-BB) enhanced phospholipase D (PLD) activity in a concentration dependent manner. Mesangial cells overexpressing the tyrosine kinase pp60c-src (c-Src) were used to determine the effect of this non transforming protooncogene on PLD activation. Overexpression of c-Src interfered with PDGF-BB-mediated activation of PLD. This modulation was dependent on the tyrosine kinase activity of c-Src, since overexpression of tyrosine kinase-negative mutants of c-Src did not affect PLD activation. No effect of c-Src overexpression was observed, when PLD was activated by ATP or guanosine 5'-3-O-(thio)triphosphate (GTP gamma S). The results indicate that the tyrosine kinase c-Src specifically interfered with PDGF-mediated but not with ATP- or GTP gamma S-mediated PLD activation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0929-7855
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
193-202
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9034964-Adenosine Triphosphate,
pubmed-meshheading:9034964-Animals,
pubmed-meshheading:9034964-Cell Division,
pubmed-meshheading:9034964-Enzyme Activation,
pubmed-meshheading:9034964-GTP-Binding Proteins,
pubmed-meshheading:9034964-Gene Expression,
pubmed-meshheading:9034964-Glomerular Mesangium,
pubmed-meshheading:9034964-Guanosine 5'-O-(3-Thiotriphosphate),
pubmed-meshheading:9034964-Humans,
pubmed-meshheading:9034964-Kinetics,
pubmed-meshheading:9034964-Phospholipase D,
pubmed-meshheading:9034964-Platelet-Derived Growth Factor,
pubmed-meshheading:9034964-Proto-Oncogene Proteins pp60(c-src),
pubmed-meshheading:9034964-Rats,
pubmed-meshheading:9034964-Transfection
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pubmed:year |
1997
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pubmed:articleTitle |
Modulation of phospholipase D stimulation in c-src transfected mesangial cells.
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pubmed:affiliation |
Medizinische Klinik IV, Universität Erlangen-Nürnberg, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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