Linked Life Data

9031260

Source:http://linkedlifedata.com/resource/pubmed/id/9031260

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-4-30
pubmed:abstractText
GABA is a likely feedback transmitter from H1 horizontal cells to cone photoreceptors in fish retinas. Spinules arise from H1 cell dendrites in light-adapted retinas, are correlated with responses attributed to feedback, and have been proposed to be the GABA release sites. We used mAb 62-3G1, an antibody against the beta 2/beta 3 subunits of the GABAA receptor complex, to visualize GABAA receptor immunoreactivity (GABAr-IR) in photoreceptors as a function of light and dark adaptation at the electron microscopical level. Regardless of adaptation, GABAr-IR was restricted to the synaptic terminals of all cones and most rods; synaptic vesicular membrane and plasma membrane, exhibited GABAr-IR. Contrary to expectations, the density of GABAr-IR was least on the plasma membrane within the invagination, regardless of the presence or absence of spinules. Dense GABAr-IR was observed on the lateral surface of cone pedicles, on cone processes proximal to the invagination, and on presumed telodendria from nearby cones. There was no difference in GABAr-IR of rod plasma membranes within or outside of the invagination or with adaptation. The only novel effect of adaptation was in regards to the density of synaptic vesicles. Cones showed a 29% increase in vesicle density with dark adaptation, whereas rods showed a 17% decrease. We conclude that all goldfish photoreceptors will be GABA-sensitive and that the sensitivity is distributed over the surface of the synaptic terminal rather than localized to within the invagination. The role of spinules in GABA release remains to be determined, but we conclude that spinules are not related to the GABA sensitivity of goldfish photoreceptors.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1059-910X
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
43-56
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Light adaptation affects synaptic vesicle density but not the distribution of GABAA receptors in goldfish photoreceptor terminals.
pubmed:affiliation
Department of Neurobiology and Behavior, State University of New York, Stony Brook 11794, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.