Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1997-3-5
pubmed:abstractText
We have previously reported the cloning of a novel cytokine, IFN-gamma-inducing factor (IGIF), which shared some biologic activities with IL-12. In this study, we analyzed the effects of murine IGIF on the activation of T cells, and compared the effects with those of IL-12. IGIF alone had no effect on the activation of T cell lines or Th1 clones, while IGIF increased the IFN-gamma production by antigen-stimulated T cell lines, but had no effect on IL-4 or IL-10 production. As reported with IL-12, IGIF served as a costimulatory factor for Th1 clones stimulated with Ag on B cell APC, immobilized anti-CD3, Con A, or IL-2 to augment IFN-gamma production and to induce IL-2R alpha-chain expression and proliferation of the Th1 clones, whereas IGIF had little or no effect on the IL-4 production and proliferation of Th2 clones stimulated with anti-CD3 or Ag. However, IGIF synergized with IL-12 to further augment the IFN-gamma production of the Th1 clones. Even in the presence of saturated amounts of IL-12, IGIF still augmented the IFN-gamma production and proliferation and enhanced the IL-2R alpha-chain expression of the Th1 clones. In contrast with IL-12, IGIF induced IL-2 production by Ag- or anti-CD3-stimulated Th1 clones. These two findings indicate that IGIF and IL-12 are utilizing different signal transduction pathways. We also found that IGIF as well as IL-12 was endogenously released through interaction between Th1 cells and spleen cell APC in the presence of specific Ag, and that it regulated IFN-gamma production. These results further suggest that IGIF may act as an immunoregulatory factor in the immune response.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
158
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1541-50
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9029088-Animals, pubmed-meshheading:9029088-Cell Line, pubmed-meshheading:9029088-Clone Cells, pubmed-meshheading:9029088-Cytokines, pubmed-meshheading:9029088-Dose-Response Relationship, Immunologic, pubmed-meshheading:9029088-Drug Synergism, pubmed-meshheading:9029088-Female, pubmed-meshheading:9029088-Interferon Inducers, pubmed-meshheading:9029088-Interferon-gamma, pubmed-meshheading:9029088-Interleukin-12, pubmed-meshheading:9029088-Interleukin-18, pubmed-meshheading:9029088-Interleukin-2, pubmed-meshheading:9029088-Lymphocyte Activation, pubmed-meshheading:9029088-Mice, pubmed-meshheading:9029088-Mice, Inbred BALB C, pubmed-meshheading:9029088-Mice, Inbred C3H, pubmed-meshheading:9029088-Mice, Inbred C57BL, pubmed-meshheading:9029088-Ovalbumin, pubmed-meshheading:9029088-Receptors, Interleukin-2, pubmed-meshheading:9029088-Th1 Cells, pubmed-meshheading:9029088-Th2 Cells
pubmed:year
1997
pubmed:articleTitle
IFN-gamma-inducing factor (IGIF) is a costimulatory factor on the activation of Th1 but not Th2 cells and exerts its effect independently of IL-12.
pubmed:affiliation
Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc., Okayama, Japan.
pubmed:publicationType
Journal Article, Comparative Study