Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1997-3-17
|
| pubmed:abstractText |
Expression of the CD4 antigen was observed on human fetal liver, fetal bone marrow (BM), and umbilical cord blood progenitors expressing high levels of CD34. Using clonal and liquid-culture assays, CD4+ CD34++ Lin- (lineage = CD3, CD8, CD10, CD14, CD15, CD16, CD19, CD20, and glycophorin A) fetal liver progenitors were found to have a greater proliferative potential than CD4- CD34++ Lin- progenitors, whereas the CD4- fraction was more enriched for erythroid progenitors. Both the CD4+ and the CD4- progenitor subpopulations also gave rise to multilineage engraftment upon transplantation into human fetal bone fragments, supportive of B-lymphoid and myeloid growth, or into human fetal thymic fragments, supportive of T-cell growth, implanted in scid/scid (SCID) mice. However, in SCID-hu mice transplanted with graded doses of donor cells ranging from 2.0 x 10(2) to 2.0 x 10(4) cells, BM reconstitution by the CD4+ fraction of CD34++ Lin- cells was more frequent than by the CD4- fraction when low numbers of cells were injected. These functional data strongly suggest that stem cells reside among CD4+ CD34++ Lin- fetal liver cells. This hypothesis was further supported by the observations that CD4+ CD34++ Lin- fetal liver cells were enriched for CDw90+ (Thy-1), CD117+ (kit), CD123+, HLA-DR+, CD7-, CD38-, CD45RA-, CD71-, CD115- (fms), and rhodamine 123(dull) cells, a phenotypic profile believed to represent fetal stem cells. Furthermore, all CD4+ CD34++ Lin- fetal liver cells also expressed CD13 and CD33.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0006-4971
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
89
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1364-75
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9028960-Animals,
pubmed-meshheading:9028960-Antigens, CD34,
pubmed-meshheading:9028960-Antigens, CD4,
pubmed-meshheading:9028960-Bone Marrow,
pubmed-meshheading:9028960-Bone Marrow Cells,
pubmed-meshheading:9028960-Cell Differentiation,
pubmed-meshheading:9028960-Cell Lineage,
pubmed-meshheading:9028960-Fetal Blood,
pubmed-meshheading:9028960-Fetal Tissue Transplantation,
pubmed-meshheading:9028960-Hematopoietic Stem Cell Transplantation,
pubmed-meshheading:9028960-Hematopoietic Stem Cells,
pubmed-meshheading:9028960-Humans,
pubmed-meshheading:9028960-Immunophenotyping,
pubmed-meshheading:9028960-Liver,
pubmed-meshheading:9028960-Mice,
pubmed-meshheading:9028960-Mice, SCID,
pubmed-meshheading:9028960-Radiation Chimera,
pubmed-meshheading:9028960-Thymus Gland,
pubmed-meshheading:9028960-Transplantation, Heterologous
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Phenotypic and functional evidence for the expression of CD4 by hematopoietic stem cells isolated from human fetal liver.
|
| pubmed:affiliation |
Human Immunology Department, DNAX Research Institute, Palo Alto, CA 94304-1104, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|