Linked Life Data

9028263

Source:http://linkedlifedata.com/resource/pubmed/id/9028263

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1997-2-26
pubmed:abstractText
Peptostreptococcus micros, an anaerobic gram-positive coccus, has been associated with periodontal and endodontic lesions, including those refractory to treatment, as well as many human polymicrobial infections in other body locations. A selective and differential medium for the primary isolation of P. micros was developed and evaluated. Columbia CNA agar, a selective medium for gram-positive cocci, was supplemented with glutathione and lead acetate (P. micros medium: PMM). P. micros has a characteristic of rapidly utilizing the reduced form of glutathione to form hydrogen sulfide, which reacts with lead acetate producing a black precipitate in the medium. When grown on PMM, P. micros can be easily identified by its typical colonial morphology and the presence of a black precipitate directly under the colony. PMM was compared for the growth of P. micros with phenylethyl alcohol agar (PEA) and Columbia base medium (CBM) with 80 strains of P. micros and 30 strains of other gram-positive cocci. All P. micros isolates tested grew and showed the typical morphology of P. micros on PMM. Using colony counts on CBM as controls, there was an average 81.8% recovery in the number of P. micros colonies on PMM, in contrast to an average 6.1% recovery on PEA. Subgingival plaque and tongue samples from 12 adult periodontitis and 6 early-onset periodontitis patients were cultured onto PMM for the isolation of P. micros. P. micros was isolated on PMM and identified biochemically and enzymatically from both adult periodontitis and early-onset periodontitis patients with higher percentages isolated from the diseased periodontal pockets of adult periodontitis patients; furthermore, this is the first isolation of P. micros from tongue samples taken from periodontally diseased patients. This medium in cultural studies will further our understanding and assist future investigations of P. micros involved in disease processes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
D
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0902-0055
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
356-61
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:9028263-Agar, pubmed-meshheading:9028263-Analysis of Variance, pubmed-meshheading:9028263-Bacteriological Techniques, pubmed-meshheading:9028263-Colony Count, Microbial, pubmed-meshheading:9028263-Culture Media, pubmed-meshheading:9028263-Dental Plaque, pubmed-meshheading:9028263-Evaluation Studies as Topic, pubmed-meshheading:9028263-Glutathione, pubmed-meshheading:9028263-Humans, pubmed-meshheading:9028263-Hydrogen Sulfide, pubmed-meshheading:9028263-Organometallic Compounds, pubmed-meshheading:9028263-Peptostreptococcus, pubmed-meshheading:9028263-Periodontitis, pubmed-meshheading:9028263-Sensitivity and Specificity, pubmed-meshheading:9028263-Staphylococcus, pubmed-meshheading:9028263-Statistics, Nonparametric, pubmed-meshheading:9028263-Streptococcus, pubmed-meshheading:9028263-Tongue
pubmed:year
1996
pubmed:articleTitle
Development and evaluation of a selective and differential medium for the primary isolation of Peptostreptococcus micros.
pubmed:affiliation
Department of Oral and Craniofacial Biological Sciences, School of Dentistry, University of Maryland at Baltimore 21201, USA.
pubmed:publicationType
Journal Article, Comparative Study