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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
1997-4-16
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pubmed:abstractText |
Transcription of the mouse vas deferens protein (MVDP) gene, a member of the aldo-keto reductase superfamily, is stimulated by androgens via the androgen responsive element (ARE) located in the proximal promoter (-111 to -97). We investigated interaction between androgens and the protein kinase C (PKC) signalling pathway. Transcriptional regulation was determined by analysis of chloramphenicol acetyltransferase (CAT). T47D cells were transiently transfected with 5' flanking MVDP DNA promoter sequences (-1804 to +41; -510 to +41 and -121 to +41) fused to the reporter (CAT) gene. Androgen-induced transcriptional activity can be enhanced from 6 (1.8 and 0.5 kb MVDP-CAT constructs) to 18 fold (0.16 kb MVDP-CAT construct), in a time and dose-dependent manner, by the PKC activator 12-o-tetradecanoylphorbol-13 acetate (TPA). A mutation in the proximal ARE abolished both androgen and TPA-dependent gene enhancement. TPA influenced minimally MMTV promoter in T47D cells and MVDP promoter in CV1 cells suggesting that the effects of the PKC activator are probably promoter and cell-specific. In contrast, activation of protein kinase A (PKA) via addition of dibutyryl-cAMP (db-cAMP) reduced androgen induction of the MVDP gene.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0303-7207
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
29
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pubmed:volume |
124
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
79-86
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9027327-Aldehyde Reductase,
pubmed-meshheading:9027327-Animals,
pubmed-meshheading:9027327-Breast Neoplasms,
pubmed-meshheading:9027327-Bucladesine,
pubmed-meshheading:9027327-Carcinoma,
pubmed-meshheading:9027327-Cell Line,
pubmed-meshheading:9027327-Cercopithecus aethiops,
pubmed-meshheading:9027327-Cyclic AMP-Dependent Protein Kinases,
pubmed-meshheading:9027327-Dihydrotestosterone,
pubmed-meshheading:9027327-Enzyme Activation,
pubmed-meshheading:9027327-Gene Expression Regulation,
pubmed-meshheading:9027327-Humans,
pubmed-meshheading:9027327-Male,
pubmed-meshheading:9027327-Mammary Tumor Virus, Mouse,
pubmed-meshheading:9027327-Mice,
pubmed-meshheading:9027327-Promoter Regions, Genetic,
pubmed-meshheading:9027327-Protein Kinase C,
pubmed-meshheading:9027327-Proteins,
pubmed-meshheading:9027327-Recombinant Fusion Proteins,
pubmed-meshheading:9027327-Signal Transduction,
pubmed-meshheading:9027327-Tetradecanoylphorbol Acetate,
pubmed-meshheading:9027327-Vas Deferens
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pubmed:year |
1996
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pubmed:articleTitle |
Protein kinase C pathway potentiates androgen-mediated gene expression of the mouse vas deferens specific aldose reductase-like protein (MVDP).
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pubmed:affiliation |
Laboratoire de Physiologie Comparée et Endocrinologie, CNRS URA 1940, Université Blaise Pascal-Clermont Ferrand II, Aubière, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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