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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1997-2-18
|
| pubmed:abstractText |
The effect of tyrosine protein kinase inhibitors on the swelling-induced chloride current (ICl-swelling) of dog atrial myocytes was studied using the whole-cell patch-clamp recording technique. Currents were measured during hyperpolarizing voltage ramps with potassium currents blocked by cesium. Osmolarity was varied using mannitol. Exposure to hypotonic solution (approximately 249 mosmol/kg) activated ICl-swelling. Hypertonic solution (approximately 363 mosmol/kg) was used to shrink swollen cells and turn off ICl-swelling. In studies on the acute effect of tyrosine protein kinase inhibitors each cell was swollen three separate times. Control, treatment, and washout ICl-swelling were compared. Genistein (50-80 microM) prevented reactivation of ICl-swelling without affecting cell size. The effect of genistein partially subsided upon washout. The effect of genistein on ICl-swelling was not mimicked by 80 microM daidzein, a related compound that does not inhibit tyrosine protein kinases. When intracellular adenosine 5'-O-(3-thiotriphosphate (ATP[gamma S]) was used, genistein did not prevent the reactivation of ICl-swelling. Intracellular ATP[gamma S] did not result in a persistent activation of ICl-swelling when cell size was returned to control. Acute exposure to 1 microM herbimycin A or 100 microM tyrphostin 51 did not prohibit the activation of ICl-swelling. A 24-h exposure to 1 microM herbimycin A did inhibit ICl-swelling. The results provide important clues regarding the activation mechanism for ICl-swelling and suggest that a tyrosine protein phosphorylation may be necessary, but not sufficient, for activation of ICl-swelling.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Benzoquinones,
http://linkedlifedata.com/resource/pubmed/chemical/Chloride Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Genistein,
http://linkedlifedata.com/resource/pubmed/chemical/Hypotonic Solutions,
http://linkedlifedata.com/resource/pubmed/chemical/Isoflavones,
http://linkedlifedata.com/resource/pubmed/chemical/Lactams, Macrocyclic,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Quinones,
http://linkedlifedata.com/resource/pubmed/chemical/adenosine 5'-O-(3-thiotriphosphate),
http://linkedlifedata.com/resource/pubmed/chemical/herbimycin
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0031-6768
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
431
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
178-85
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:9026777-Adenosine Triphosphate,
pubmed-meshheading:9026777-Animals,
pubmed-meshheading:9026777-Benzoquinones,
pubmed-meshheading:9026777-Cell Size,
pubmed-meshheading:9026777-Chloride Channels,
pubmed-meshheading:9026777-Dogs,
pubmed-meshheading:9026777-Electrophysiology,
pubmed-meshheading:9026777-Enzyme Inhibitors,
pubmed-meshheading:9026777-Genistein,
pubmed-meshheading:9026777-Heart,
pubmed-meshheading:9026777-Hypotonic Solutions,
pubmed-meshheading:9026777-Isoflavones,
pubmed-meshheading:9026777-Lactams, Macrocyclic,
pubmed-meshheading:9026777-Membrane Potentials,
pubmed-meshheading:9026777-Myocardium,
pubmed-meshheading:9026777-Patch-Clamp Techniques,
pubmed-meshheading:9026777-Phosphorylation,
pubmed-meshheading:9026777-Protein-Tyrosine Kinases,
pubmed-meshheading:9026777-Quinones
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Tyrosine protein kinase inhibitors prevent activation of cardiac swelling-induced chloride current.
|
| pubmed:affiliation |
Department of Pharmacology, Columbia University, New York, NY 10032, USA.
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|