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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1997-4-7
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pubmed:abstractText |
We describe a procedure for the enzymatic synthesis of labeled or unlabeled GDP-D-arabinopyranoside. This method uses two enzymes purified from pig kidney: an L-fucokinase and a GDP-L-fucose pyrophosphorylase. The isolated GDP-D-[3H]arabinose served as a precursor for arabinose addition to lipophosphoglycan (LPG) of Leishmania major, using a parasite membrane fraction as the source of arabinosyltransferase. The procedures described provide a useful means for obtaining radiolabeled GDP-D-arabinopyranoside to study synthesis of D-arabinopyranoside-containing glycoconjugates.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0003-2697
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
244
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
321-7
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9025949-Animals,
pubmed-meshheading:9025949-Arabinose,
pubmed-meshheading:9025949-Chromatography, Gel,
pubmed-meshheading:9025949-Crithidia,
pubmed-meshheading:9025949-Guanosine Diphosphate Sugars,
pubmed-meshheading:9025949-Kidney,
pubmed-meshheading:9025949-Leishmania major,
pubmed-meshheading:9025949-Nucleotidyltransferases,
pubmed-meshheading:9025949-Phosphotransferases (Alcohol Group Acceptor),
pubmed-meshheading:9025949-Sugar Phosphates,
pubmed-meshheading:9025949-Swine
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pubmed:year |
1997
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pubmed:articleTitle |
Synthesis and utilization of GDP-D-arabinopyranoside.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, University of Arkanasas for Medical Sciences, Little Rock 72211, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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