Linked Life Data

9025260

Source:http://linkedlifedata.com/resource/pubmed/id/9025260

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1997-6-5
pubmed:abstractText
The use of (micro) liquid chromatography electrospray mass spectrometry (LC-ES-MS) was investigated as potential technique for the determination of the high-molecular-mass protein toxin Staphylococcal Enterotoxin B (SEB). The molecular mass was determined (28,366.3 +/- 1.1) by flow injection analysis and micro-LC-MS with a TSK-gel Phenyl-5PW column packing. Both methods allowed molecular-mass determination of SEB at levels down to 3 pmol/ml. Additional evidence of identification was obtained by detecting the presence of a disulfide bridge by the addition of 2-mercaptoethanol and by tryptic digestion. Collision induced dissociation spectra were recorded from the major tryptic fragments resulting in a sufficient number of sequence ions to allow for the determination of the amino acid sequence. On the analysis of the tryptic digests with C18 reversed-phase columns the use of micro-LC-MS-MS resulted in an 30-40-fold increase in sensitivity as compared with conventional-size LC-MS-MS.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
3
pubmed:volume
757
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
51-64
pubmed:dateRevised
2009-1-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Determination of staphylococcal enterotoxin B by on-line (micro) liquid chromatography-electrospray mass spectrometry.
pubmed:affiliation
TNO Prins Maurits Laboratory, Rijswijk, The Netherlands.
pubmed:publicationType
Journal Article, Comparative Study