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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
|
pubmed:dateCreated |
1997-4-3
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pubmed:abstractText |
We describe a semiquantitative method to measure hepatitis C virus (HCV) viral particle numbers, by carrying out reverse-transcription polymerase chain reaction (RT-PCR) on serial dilutions of serum samples. The virus concentrations measured were 10(3)-10(6) viral particles ml-1 of serum. The method described is relatively quick, and the only required manipulation is dilution of the serum. An optimal RT-PCR method is used for diluted and undiluted samples.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Dec
|
pubmed:issn |
0890-8508
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
10
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
477-80
|
pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9025088-Hepacivirus,
pubmed-meshheading:9025088-Hepatitis C,
pubmed-meshheading:9025088-Humans,
pubmed-meshheading:9025088-Indicator Dilution Techniques,
pubmed-meshheading:9025088-Polymerase Chain Reaction,
pubmed-meshheading:9025088-RNA, Viral,
pubmed-meshheading:9025088-Viral Load,
pubmed-meshheading:9025088-Viremia
|
pubmed:year |
1996
|
pubmed:articleTitle |
A simplified semiquantitative determination of hepatitis C virus genome molecules by the end-point dilution method.
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pubmed:affiliation |
LCL Laboratory, Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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