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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
|
| pubmed:dateCreated |
1997-10-22
|
| pubmed:databankReference | |
| pubmed:abstractText |
Genomic DNA was isolated from adult Strongylus asini collected from zebra. The second ribosomal transcribed spacer (ITS-2) was amplified and sequenced using polymerase chain reaction (PCR) based techniques. The DNA sequence was compared with previously published data for 3 related Strongylus species. A PCR-linked restriction fragment length polymorphism method allowed the 4 species to be differentiated unequivocally. The ITS-2 sequence of S. asini was found to be more similar to those of S. edentatus (87.1%) and S. equinus (95.3%) than to that of S vulgaris (73.9%). This result confirms that S. Asini and S vulgaris represent separate species and supports the retention of the 4 species within 1 genus.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0020-7519
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
26
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1407-11
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9024894-Animals,
pubmed-meshheading:9024894-Base Sequence,
pubmed-meshheading:9024894-DNA, Ribosomal,
pubmed-meshheading:9024894-DNA Primers,
pubmed-meshheading:9024894-Evolution, Molecular,
pubmed-meshheading:9024894-Female,
pubmed-meshheading:9024894-Male,
pubmed-meshheading:9024894-Molecular Sequence Data,
pubmed-meshheading:9024894-Namibia,
pubmed-meshheading:9024894-Perissodactyla,
pubmed-meshheading:9024894-Phylogeny,
pubmed-meshheading:9024894-Polymerase Chain Reaction,
pubmed-meshheading:9024894-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:9024894-Restriction Mapping,
pubmed-meshheading:9024894-Sequence Alignment,
pubmed-meshheading:9024894-Sequence Homology, Nucleic Acid,
pubmed-meshheading:9024894-South Africa,
pubmed-meshheading:9024894-Strongylus
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Strongylus asini (Nematoda, Strongyloidea): genetic relationships with other Strongylus species determined by ribosomal DNA.
|
| pubmed:affiliation |
Department of Veterinary Science, University of Melbourne, Werribee, Victoria, Australia.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|