|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1997-3-10
|
|
pubmed:abstractText |
Kaposi sarcoma (KS) is the most common tumor associated with HIV-1 infection and develops in nearly 30% of cases. The principal features of this tumor are abnormal vascularization and the proliferation of endothelial cells and spindle (tumor) cells. KS-derived spindle cells induce vascular lesions and display enhanced vascular permeability when inoculated subcutaneously in the nude mouse. This finding suggests that angiogenesis and capillary permeability play a central role in the development and progression of KS. In this study, we show that AIDS-KS cell lines express higher levels of vascular endothelial growth factor/vascular permeability factor (VEGF/VGF) than either human umbilical vein endothelial cells or human aortic smooth muscle cells. AIDS-KS cells and primary tumor tissues also expressed high levels of Flt-1 and KDR, the receptors for VEGF, while the normal skin of the same patients did not show any expression. We further demonstrate that VEGF antisense oligonucleotides AS-1 and AS-3 specifically block VEGF mRNA and protein production and inhibit KS cell growth in a dose-dependent manner. Furthermore, growth of KS cells in nude mice was specifically inhibited by VEGF antisense oligonucleotides. These results show that VEGF is an autocrine growth factor for AIDS-KS cells. To our knowledge, this is the first report that shows that VEGF acts as a growth stimulator in a human tumor. Inhibitors of VEGF or its cognate receptors may thus be candidates for therapeutic intervention.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-1312256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-1372863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-1417831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-1431144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-1542792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-1567395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-1711045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-1925582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-1940805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-2184372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-2440339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-2446517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-2459779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-2472743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-2479986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-2479987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-2643161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-2735925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-3262925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-3265151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-3901851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-3990755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-7629884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-7681362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-7780129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-7811548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-7814392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-7824266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-7935812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-8030759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-8100400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-8126087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-8217343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-8264155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-8374885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-8416373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-8644848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9023368-8669484
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Endothelial Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphokines,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Vascular Endothelial...,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor A,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor...,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factors
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0027-8424
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
4
|
|
pubmed:volume |
94
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
979-84
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:9023368-Humans,
pubmed-meshheading:9023368-Animals,
pubmed-meshheading:9023368-Mice,
pubmed-meshheading:9023368-Sarcoma, Kaposi,
pubmed-meshheading:9023368-Neoplasm Transplantation,
pubmed-meshheading:9023368-Tumor Cells, Cultured,
pubmed-meshheading:9023368-Endothelium, Vascular,
pubmed-meshheading:9023368-Muscle, Smooth, Vascular,
pubmed-meshheading:9023368-Lymphokines,
pubmed-meshheading:9023368-Mice, Nude,
pubmed-meshheading:9023368-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:9023368-Vascular Endothelial Growth Factor A,
pubmed-meshheading:9023368-Vascular Endothelial Growth Factors,
pubmed-meshheading:9023368-Receptor Protein-Tyrosine Kinases,
pubmed-meshheading:9023368-Endothelial Growth Factors,
pubmed-meshheading:9023368-Receptors, Vascular Endothelial Growth Factor,
pubmed-meshheading:9023368-Proto-Oncogene Proteins,
pubmed-meshheading:9023368-AIDS-Related Opportunistic Infections,
pubmed-meshheading:9023368-Receptors, Growth Factor,
pubmed-meshheading:9023368-Oligonucleotides, Antisense,
pubmed-meshheading:9023368-Vascular Endothelial Growth Factor Receptor-1
|
