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rdf:type |
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lifeskim:mentions |
umls-concept:C0034790,
umls-concept:C0035253,
umls-concept:C0039194,
umls-concept:C0205263,
umls-concept:C0332120,
umls-concept:C0376315,
umls-concept:C0425152,
umls-concept:C1332709,
umls-concept:C1514485,
umls-concept:C1515655,
umls-concept:C1533691
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pubmed:issue |
1
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pubmed:dateCreated |
1997-3-5
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pubmed:abstractText |
JJ316 and JJ319 are rat CD28-specific monoclonal antibodies (mAb) of the gamma1 kappa isotype with identical co-stimulatory potency. At a concentration 100-1000-fold higher than that required for co-stimulation, JJ316, but not JJ319 induces massive proliferation of all T cell subsets in vitro without T cell receptor (TCR) triggering. "Direct" stimulation by JJ316 is fully blocked by JJ319, indicating that it is not due to cross-reactivity of JJ316 with the TCR complex or other activating receptors. JJ316 binds much more slowly to primary T cells than JJ319, whereas both antibodies bind with similar kinetics to CD28-transfected L-929 cells, suggesting that JJ316 binding to T cells requires redistribution or a conformational change of CD28. In vivo, JJ316 but not JJ319 induces rapid and transient proliferation of most CD4 T cells and, indirectly, of B cells. These data show that TCR engagement is not an absolute prerequisite either in vitro or in vivo for the induction of T cell proliferation through CD28 and suggest that mAb JJ316 is able to stimulate resting T cells directly by recruiting CD28 molecules from an inactive to an active form.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD28,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD80,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD86,
http://linkedlifedata.com/resource/pubmed/chemical/Cd86 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0014-2980
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
239-47
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9022025-Animals,
pubmed-meshheading:9022025-Antibodies, Monoclonal,
pubmed-meshheading:9022025-Antigens, CD,
pubmed-meshheading:9022025-Antigens, CD28,
pubmed-meshheading:9022025-Antigens, CD80,
pubmed-meshheading:9022025-Antigens, CD86,
pubmed-meshheading:9022025-Cell Division,
pubmed-meshheading:9022025-Female,
pubmed-meshheading:9022025-Kinetics,
pubmed-meshheading:9022025-Lymph Nodes,
pubmed-meshheading:9022025-Lymphocyte Activation,
pubmed-meshheading:9022025-Lymphocyte Subsets,
pubmed-meshheading:9022025-Male,
pubmed-meshheading:9022025-Membrane Glycoproteins,
pubmed-meshheading:9022025-Rats,
pubmed-meshheading:9022025-Rats, Inbred Lew,
pubmed-meshheading:9022025-Receptors, Antigen, T-Cell,
pubmed-meshheading:9022025-Signal Transduction,
pubmed-meshheading:9022025-Spleen,
pubmed-meshheading:9022025-T-Lymphocytes
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pubmed:year |
1997
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pubmed:articleTitle |
CD28-mediated induction of proliferation in resting T cells in vitro and in vivo without engagement of the T cell receptor: evidence for functionally distinct forms of CD28.
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pubmed:affiliation |
Institute for Virology and Immunobiology, University of Würzburg, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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