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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1997-3-31
|
| pubmed:abstractText |
Free/pedicled myocutaneous flaps used as functional replacement after radical dissection of advanced stage squamous cell carcinomas of the oral cavity/oropharynx were examined by immunohistochemistry (APAAP-technique). Biopsies from eight patients were taken at the time of surgery and at 3 and 5 months post-operatively. Fifteen monoclonal antibodies were used to detect surface antigens as markers of phenotypic changes of immune competent cells. In post-operative biopsies all antigens investigated increased significantly. Significantly higher numbers of CD45RO+ (P < 0.01) and CD45RA+ (P < 0.001) leukocytes were detectable. The majority of these leukocytes were TcR alpha/beta +/CD3+ T-cells, which increased in the CD4 (P < 0.05) and the CD8 (P < 0.001) subset. In addition, B-cells (P < 0.05), granulocytes (P < 0.05), NK cells (CD16+ lymphocytic cells; P < 0.05) and mature macrophages (25F9+cells; P < 0.01) were increased. Intra- and subepidermally a significantly (P < 0.01) higher number of dendritic-/Langerhans cells (CD1a+) was detectable. In post-operative biopsies, the activation-associated antigens ICAM-1, VCAM and HLA-DR were expressed on significantly more mononuclear-/endothelial cells and on keratinocytes. Our findings indicate that the myocutaneous flaps still contained cells with immunological capacities.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
D
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0904-2512
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
26
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
29-35
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:9021549-Aged,
pubmed-meshheading:9021549-Aged, 80 and over,
pubmed-meshheading:9021549-Antigens, CD,
pubmed-meshheading:9021549-Carcinoma, Squamous Cell,
pubmed-meshheading:9021549-HLA-DR Antigens,
pubmed-meshheading:9021549-Humans,
pubmed-meshheading:9021549-Immunoenzyme Techniques,
pubmed-meshheading:9021549-Immunophenotyping,
pubmed-meshheading:9021549-Killer Cells, Natural,
pubmed-meshheading:9021549-Langerhans Cells,
pubmed-meshheading:9021549-Leukocytes,
pubmed-meshheading:9021549-Lymphocyte Subsets,
pubmed-meshheading:9021549-Macrophages,
pubmed-meshheading:9021549-Male,
pubmed-meshheading:9021549-Middle Aged,
pubmed-meshheading:9021549-Mouth Floor,
pubmed-meshheading:9021549-Mouth Mucosa,
pubmed-meshheading:9021549-Mouth Neoplasms,
pubmed-meshheading:9021549-Surgical Flaps
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Myocutaneous flaps in patients with head and neck cancer retain their immunological capacities in an activated functional state.
|
| pubmed:affiliation |
Department of Oto-Rhino-Laryngology, University of Vienna, Austria.
|
| pubmed:publicationType |
Journal Article
|