Linked Life Data

9020464

Source:http://linkedlifedata.com/resource/pubmed/id/9020464

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-3-7
pubmed:abstractText
We obtained intervertebral discs with cartilage endplates and underlying cancellous bone at operation from patients with degenerative disc disease and then used immunohistochemical techniques to localise the nerves and nerve endings in the specimens. We used antibodies for the ubiquitous neuronal protein gene product 9.5 (PGP 9.5). Immunoreactivity to neuropeptide Y was used to identify autonomic nerves and calcitonin gene-related peptide (CGRP) and substance P to identify sensory nerves. Blood vessels were identified by immunoreactivity with platelet-endothelial cell-adhesion molecule (CD31; PECAM). In a control group with no known history of chronic back pain, nerve fibres immunoreactive to PGP 9.5 and neuropeptide Y were most closely related to blood vessels, with occasional substance P and CGRP immunoreactivity. In patients with severe back pain and markedly reduced disc height, proliferation of blood vessels and accompanying nerve fibres was observed in the endplate region and underlying vertebral bodies. Many of these nerves were immunoreactive to substance P or CGRP, and in addition, substance P- and CGRP-immunoreactive nociceptors were seen unrelated to blood vessels. Quantification by image analysis showed a marked increase in CGRP-containing sensory nerve fibres compared with normal control subjects. We speculate that a chemotactic response to products of disc breakdown is responsible for the proliferation of vascularity and CGRP-containing sensory nerves found in the endplate region and vertebral body adjacent to degenerate discs. The neuropeptides substance P and CGRP have potent vasodilatory as well as pain-transmitting effects. The increase in sensory nerve endings suggests increase in blood flow, perhaps as an attempt to augment the nutrition of the degenerate disc. The increase in the density of sensory nerves, and the presence of endplate cartilage defects, strongly suggest that the endplates and vertebral bodies are sources of pain; this may explain the severe pain on movement experienced by some patients with degenerative disc disease.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0301-620X
pubmed:author
pubmed:issnType
Print
pubmed:volume
79
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
147-53
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:9020464-Adolescent, pubmed-meshheading:9020464-Adult, pubmed-meshheading:9020464-Aged, pubmed-meshheading:9020464-Antigens, CD31, pubmed-meshheading:9020464-Calcitonin Gene-Related Peptide, pubmed-meshheading:9020464-Cartilage, pubmed-meshheading:9020464-Female, pubmed-meshheading:9020464-Humans, pubmed-meshheading:9020464-Immunohistochemistry, pubmed-meshheading:9020464-Intervertebral Disc, pubmed-meshheading:9020464-Intervertebral Disc Displacement, pubmed-meshheading:9020464-Male, pubmed-meshheading:9020464-Middle Aged, pubmed-meshheading:9020464-Nerve Endings, pubmed-meshheading:9020464-Nerve Tissue Proteins, pubmed-meshheading:9020464-Neurons, Afferent, pubmed-meshheading:9020464-Neuropeptide Y, pubmed-meshheading:9020464-Nociceptors, pubmed-meshheading:9020464-Substance P, pubmed-meshheading:9020464-Sympathetic Nervous System, pubmed-meshheading:9020464-Thiolester Hydrolases, pubmed-meshheading:9020464-Ubiquitin Thiolesterase
pubmed:year
1997
pubmed:articleTitle
Sensory and sympathetic innervation of the vertebral endplate in patients with degenerative disc disease.
pubmed:affiliation
Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't