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rdf:type |
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lifeskim:mentions |
umls-concept:C0012472,
umls-concept:C0033268,
umls-concept:C0033532,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0040061,
umls-concept:C0181586,
umls-concept:C0205263,
umls-concept:C0206190,
umls-concept:C0243769,
umls-concept:C0387583,
umls-concept:C0558295,
umls-concept:C1548789
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pubmed:issue |
1
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pubmed:dateCreated |
1997-2-13
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pubmed:abstractText |
Rat peritoneal macrophages were stimulated with lipopolysaccaride (LPS) for various periods and their ability to convert exogenous arachidonic acid to various prostanoids was examined. Unstimulated cells, which expressed cyclooxygenase (COX)-1 but not COX-2, produced thromboxane (TX) B2 > prostaglandin (PG) D2 > PGE2, whereas cells stimulated for 6-12 h with LPS exhibited marked increase in conversion to PGE2, which paralleled COX-2 induction, with minimal change in conversion to TXB2 and PGD2. Pharmacological studies showed that formation of PGE2 was mediated predominantly by COX-2, PGD2 by COX-1, and TXB2 by both COX-1 and COX-2 depending upon the timing of LPS stimulation. Measurement of the conversion of exogenous PGH2 to each prostanoid in cell lysates demonstrated LPS-dependent increase in PGE2 synthase activity that was degenerated by pretreatment with actinomycin D or cycloheximide. Thus, concordant induction of terminal PGE2 synthase with COX-2 leads to the preferred production of PGE2 to TXB2 and PGD2 by LPS-stimulated macrophages.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cycloheximide,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2 Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Dactinomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Indomethacin,
http://linkedlifedata.com/resource/pubmed/chemical/Intramolecular Oxidoreductases,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Isomerases,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/N-(2-cyclohexyloxy-4-nitrophenyl)met...,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrobenzenes,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin D2,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin-Endoperoxide Synthases,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfonamides,
http://linkedlifedata.com/resource/pubmed/chemical/Thromboxane B2,
http://linkedlifedata.com/resource/pubmed/chemical/prostaglandin-E synthase
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
230
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
110-4
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9020023-Animals,
pubmed-meshheading:9020023-Cells, Cultured,
pubmed-meshheading:9020023-Cycloheximide,
pubmed-meshheading:9020023-Cyclooxygenase 2,
pubmed-meshheading:9020023-Cyclooxygenase 2 Inhibitors,
pubmed-meshheading:9020023-Cyclooxygenase Inhibitors,
pubmed-meshheading:9020023-Dactinomycin,
pubmed-meshheading:9020023-Dinoprostone,
pubmed-meshheading:9020023-Enzyme Induction,
pubmed-meshheading:9020023-Indomethacin,
pubmed-meshheading:9020023-Intramolecular Oxidoreductases,
pubmed-meshheading:9020023-Isoenzymes,
pubmed-meshheading:9020023-Isomerases,
pubmed-meshheading:9020023-Kinetics,
pubmed-meshheading:9020023-Lipopolysaccharides,
pubmed-meshheading:9020023-Macrophage Activation,
pubmed-meshheading:9020023-Macrophages, Peritoneal,
pubmed-meshheading:9020023-Male,
pubmed-meshheading:9020023-Nitrobenzenes,
pubmed-meshheading:9020023-Prostaglandin D2,
pubmed-meshheading:9020023-Prostaglandin-Endoperoxide Synthases,
pubmed-meshheading:9020023-Rats,
pubmed-meshheading:9020023-Rats, Sprague-Dawley,
pubmed-meshheading:9020023-Sulfonamides,
pubmed-meshheading:9020023-Thromboxane B2
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pubmed:year |
1997
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pubmed:articleTitle |
Concordant induction of prostaglandin E2 synthase with cyclooxygenase-2 leads to preferred production of prostaglandin E2 over thromboxane and prostaglandin D2 in lipopolysaccharide-stimulated rat peritoneal macrophages.
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pubmed:affiliation |
Department of Pharmacology, School of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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