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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1997-3-14
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pubmed:abstractText |
Three oligonucleotide primers were used in a polymerase chain reaction (PCR) assay for the simultaneous amplification of regions of the invasive plasmid antigen (ipaH) of Shigella spp., flagellin gene (flaA) of Campylobacter spp., and heat-labile enterotoxin (LT) of enterotoxigenic Escherichia coli (ETEC). The multiplex assay was performed using DNA extracted by a chaotropic method directly from diarrhoeal stools. The diagnostic efficacy of the assay was analyzed by agarose gel electrophoresis. This assay shows a novel approach for the diagnosis of diarrhoea caused by Shigella spp., ETEC, and Campylobacter spp.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0253-8768
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
207-10
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9019016-Campylobacter,
pubmed-meshheading:9019016-DNA, Bacterial,
pubmed-meshheading:9019016-DNA Primers,
pubmed-meshheading:9019016-Escherichia coli,
pubmed-meshheading:9019016-Feces,
pubmed-meshheading:9019016-Humans,
pubmed-meshheading:9019016-Polymerase Chain Reaction,
pubmed-meshheading:9019016-Shigella
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pubmed:year |
1996
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pubmed:articleTitle |
Detection of enterotoxigenic Escherichia coli, Shigella and Campylobacter spp. by multiplex PCR assay.
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pubmed:affiliation |
Enteric Microbiology Branch, U.S. Naval Medical Research Unit No. 3, Cairo, Egypt. namru3@centcom.dsaa.osd.mil
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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