Linked Life Data

9018073

Source:http://linkedlifedata.com/resource/pubmed/id/9018073

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1997-2-24
pubmed:databankReference
pubmed:abstractText
The ecdysteroid UDP-glucosyltransferase gene (egt) of Spodoptera littoralis multicapsid nucleopolyhedrovirus (SpliMNPV) is a homologue of the Autographa californica MNPV (AcMNPV) egt gene, which has been found to block insect moulting. Infection of larvae with an egt-deleted AcMNPV resulted in enhanced mortality as compared to infection with the wild-type virus. Consequently, deletion of an egt gene has been proposed as a tempting approach for enhancing the insecticidal properties of baculoviruses. In a previous report we described the mapping and sequencing of the SpliMNPV egt gene. Here we use time-course Northern blot and biochemical analyses to show the production of egt transcripts and protein. The SpliMNPV egt transcription start sites were mapped to 22 and 25 nucleotides downstream of the TATA box by primer extension. Transient expression assays of chimeric egt promoter-chloramphenicol acetyltransferase (cat) reporter gene constructs revealed low promoter activity that was transactivated by AcMNPV immediate-early viral protein IE-1.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-1317
pubmed:author
pubmed:issnType
Print
pubmed:volume
78 ( Pt 2)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
487-91
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Transcriptional analysis and promoter activity of the Spodoptera littoralis multicapsid nucleopolyhedrovirus ecdysteroid UDP-glucosyltransferase gene.
pubmed:affiliation
Department of Entomology, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't