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rdf:type |
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lifeskim:mentions |
umls-concept:C0006556,
umls-concept:C0009015,
umls-concept:C0009219,
umls-concept:C0017262,
umls-concept:C0043393,
umls-concept:C0064644,
umls-concept:C0185117,
umls-concept:C0205245,
umls-concept:C1123020,
umls-concept:C1413892,
umls-concept:C2911684
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pubmed:issue |
2
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pubmed:dateCreated |
1997-3-5
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pubmed:abstractText |
Screening of a wheat cDNA library with an heterologous CYP81B1 probe from Helianthus tuberosus led to the isolation of a partial cDNA coding a protein with all the characteristics of a typical P450 with high homology (32-39% identity) to the fungal and mammalian CYP51s. Extensive screening of several wheat cDNA libraries isolated a longer cDNA (W516) coding a peptide of 453 amino acids. Alignment of W516 with other P450 sequences revealed that it was missing a segment corresponding to the N-terminal membrane anchor of the protein. The corresponding segment from the yeast lanosterol 14alpha-demethylase was linked to the partial wheat cDNA and the chimera expressed in Saccharomyces cerevisiae. Compared to microsomes from control yeasts, membranes of yeast expressing the chimera catalysed 14alpha-demethylation of obtusifoliol with an increased efficiency relative to lanosterol demethylase activity. W516 is thus a plant member of the most ancient and conserved P450 family, CYP51.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cholestadienols,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Lanosterol,
http://linkedlifedata.com/resource/pubmed/chemical/Oxidoreductases,
http://linkedlifedata.com/resource/pubmed/chemical/Phytosterols,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sterol 14-Demethylase,
http://linkedlifedata.com/resource/pubmed/chemical/obtusifoliol
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
230
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
381-5
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:9016788-Amino Acid Sequence,
pubmed-meshheading:9016788-Base Sequence,
pubmed-meshheading:9016788-Cholestadienols,
pubmed-meshheading:9016788-Cloning, Molecular,
pubmed-meshheading:9016788-Cytochrome P-450 Enzyme System,
pubmed-meshheading:9016788-DNA, Complementary,
pubmed-meshheading:9016788-DNA Primers,
pubmed-meshheading:9016788-Evolution, Molecular,
pubmed-meshheading:9016788-Gene Library,
pubmed-meshheading:9016788-Lanosterol,
pubmed-meshheading:9016788-Microsomes,
pubmed-meshheading:9016788-Molecular Sequence Data,
pubmed-meshheading:9016788-Oxidoreductases,
pubmed-meshheading:9016788-Phylogeny,
pubmed-meshheading:9016788-Phytosterols,
pubmed-meshheading:9016788-Polymerase Chain Reaction,
pubmed-meshheading:9016788-Recombinant Fusion Proteins,
pubmed-meshheading:9016788-Saccharomyces cerevisiae,
pubmed-meshheading:9016788-Sequence Homology, Amino Acid,
pubmed-meshheading:9016788-Sterol 14-Demethylase,
pubmed-meshheading:9016788-Substrate Specificity,
pubmed-meshheading:9016788-Triticum
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pubmed:year |
1997
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pubmed:articleTitle |
Cloning and functional expression in yeast of a cDNA coding for an obtusifoliol 14alpha-demethylase (CYP51) in wheat.
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pubmed:affiliation |
Department of Cellular and Molecular Enzymology, Institute of Plant Molecular Biology, CNRS UPR 406, Strasbourg, France.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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