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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
|
| pubmed:dateCreated |
1997-4-15
|
| pubmed:abstractText |
Sequence analysis was used to design a pair of degenerate oligonucleotide primers that amplified a 1.6-2.1 kbp fragment from the 3' end of the genome (virion protein gene and part of the NIb gene) of 17 species of the Potyviridae ('potyvirids'); 11 potyviruses, 2 bymoviruses, 2 macluraviruses, an ipomovirus and a rymovirus. The 'potyvirid primer 1' hybridizes to the 3' terminal poly-A region of the genome, and 'potyvirid primer 2' to the genomic region encoding the-GNNSGQ-motif of the NIb protein. Database searches showed that the potyvirid 2 primer is specific for potyvirids. Associated analyses indicated that the published amino acid sequence of part of the wheat streak mosaic rymovirus NIb protein is probably incorrect in part.
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| pubmed:commentsCorrections | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0166-0934
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
63
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
9-16
|
| pubmed:dateRevised |
2000-12-18
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| pubmed:meshHeading |
pubmed-meshheading:9015271-Amino Acid Sequence,
pubmed-meshheading:9015271-Base Sequence,
pubmed-meshheading:9015271-DNA Primers,
pubmed-meshheading:9015271-Genome, Viral,
pubmed-meshheading:9015271-Molecular Sequence Data,
pubmed-meshheading:9015271-Polymerase Chain Reaction,
pubmed-meshheading:9015271-Potyvirus
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
A primer pair for amplifying part of the genome of all potyvirids by RT-PCR.
|
| pubmed:affiliation |
Research School of Biological Sciences, Australian National University, Canberra. gibbs@rsbs-central.anu.edu.au
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| pubmed:publicationType |
Journal Article
|