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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1997-2-20
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pubmed:abstractText |
The human monocytic cell lines MUTZ-3 and MONO-MAC-6 express the lipopolysaccharide (LPS) receptor CD14. Paralleling the situation in peripheral blood monocytes (PBMo), recombinant human interleukin-4 (IL-4) down-regulated the expression of CD14 on the cell surface of MUTZ-3, but not that of MONO-MAC-6 cells. In addition, preincubation with IL-4 prevented the LPS-induced up-regulation of IL-1 beta mRNA levels in MUTZ-3, but not in MONO-MAC-6 cells. We examined whether the differential responsiveness of the cell lines was due to the missing expression of the IL-4 receptor (IL-4R) alpha or gamma c chain in MONO-MAC-6 cells. Flow cytometric and immunoprecipitation analysis revealed expression of both IL-4R chains in both cell lines. In addition, short-term stimulation with IL-4 induced tyrosine-phosphorylation of the gamma c chain. As both cell lines also expressed signal transducer and activator of transcription 6 (STAT 6), our data suggested that the differential reaction patterns of MUTZ-3 and MONO-MAC-6 cells were not due to a generally defective IL-4R complex. Interestingly, long-term (48 hr) treatment with LPS rendered MONO-MAC-6 cells sensitive to IL-4. LPS up-regulated expression of monocyte-specific esterase (MSE) mRNA as well as CD14 protein in MONO-MAC-6 cells; both effects were inhibited by IL-4. This stimulation was not paralleled by an increase of IL-4R mRNA or protein expression supporting the above hypothesis of a constitutively present and active IL-4R. We discuss possible causes for the differential reaction patterns of MUTZ-3 and MONO-MAC-6 cells to IL-4.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9014829-1701391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9014829-1717365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9014829-1724447,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9014829-1725131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9014829-2119829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9014829-2785566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9014829-7533199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9014829-7535338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9014829-7549895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9014829-7612228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9014829-8085155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9014829-8228249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9014829-8372354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9014829-8422459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9014829-8667638
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD14,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
89
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
606-12
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9014829-Antigens, CD,
pubmed-meshheading:9014829-Antigens, CD14,
pubmed-meshheading:9014829-Blotting, Western,
pubmed-meshheading:9014829-Cell Line,
pubmed-meshheading:9014829-Flow Cytometry,
pubmed-meshheading:9014829-Humans,
pubmed-meshheading:9014829-Immunophenotyping,
pubmed-meshheading:9014829-Interleukin-4,
pubmed-meshheading:9014829-Lipopolysaccharides,
pubmed-meshheading:9014829-Models, Immunological,
pubmed-meshheading:9014829-Monocytes,
pubmed-meshheading:9014829-Polymerase Chain Reaction,
pubmed-meshheading:9014829-Receptors, Interleukin,
pubmed-meshheading:9014829-Receptors, Interleukin-4,
pubmed-meshheading:9014829-Recombinant Proteins
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pubmed:year |
1996
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pubmed:articleTitle |
MUTZ-3, a monocytic model cell line for interleukin-4 and lipopolysaccharide studies.
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pubmed:affiliation |
DSMZ, German Collection of microorganisms and Cell Cultures, Braunschweig, Germany.
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pubmed:publicationType |
Journal Article,
Comparative Study
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