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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1997-3-6
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pubmed:abstractText |
To determine whether formation of the stable complex between a serpin and a target proteinase involves a major translocation of the proteinase from its initial position in the noncovalent Michaelis complex, we have used fluorescence resonance energy transfer to measure the separation between fluorescein attached to a single cysteine on the serpin and tetramethylrhodamine conjugated to the proteinase. The interfluorophore separation was determined for the noncovalent Michaelis-like complex formed between alpha 1-proteinase inhibitor (Pittsburgh variant) and anhydrotrypsin and for the stable complex between the same serpin and trypsin. A difference in separation between the two fluorophores of approximately 21 A was found for the two types of complex. This demonstrates a major movement of the proteinase in going from the initial noncovalent encounter complex to the kinetically stable complex. The change in interfluorophore separation is most readily understood in terms of movement of the proteinase from the reactive center end of the serpin toward the distal end, as the covalently attached reactive center loop inserts into beta-sheet A of the serpin.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9012804-14240539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9012804-1888745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9012804-1930157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9012804-2191958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9012804-2474530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9012804-3484756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9012804-4855551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9012804-5064765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9012804-5263760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9012804-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9012804-6604220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9012804-6989830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9012804-7037776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9012804-7479695,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9012804-7495819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9012804-7592687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9012804-7613461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9012804-7795518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9012804-8031789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9012804-8347575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9012804-8530349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9012804-8530403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9012804-8652540
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
21
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pubmed:volume |
94
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
453-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9012804-Energy Transfer,
pubmed-meshheading:9012804-Humans,
pubmed-meshheading:9012804-Macromolecular Substances,
pubmed-meshheading:9012804-Motion,
pubmed-meshheading:9012804-Protein Conformation,
pubmed-meshheading:9012804-Rhodamines,
pubmed-meshheading:9012804-Serpins,
pubmed-meshheading:9012804-Spectrometry, Fluorescence,
pubmed-meshheading:9012804-Trypsin,
pubmed-meshheading:9012804-Water,
pubmed-meshheading:9012804-alpha 1-Antitrypsin
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pubmed:year |
1997
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pubmed:articleTitle |
Major proteinase movement upon stable serpin-proteinase complex formation.
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pubmed:affiliation |
Department of Biochemistry, University of Illinois at Chicago 60612, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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