Linked Life Data

9011387

Source:http://linkedlifedata.com/resource/pubmed/id/9011387

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-2-6
pubmed:abstractText
Myenteric neurons projecting to the mucosa of the guinea pig proximal colon were identified using the combination of a neuronal tracing method and immunohistochemical techniques. The tracer DiI (1, 1'didodecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate) was applied onto the mucosa of a specimen of proximal colon which was then placed in organotypic culture to allow retrograde transport of the dye. After culture, the myenteric plexus was stained with antisera raised against choline acetyltransferase (ChAT) and calbindin (Calb). Of the myenteric neurons labeled with DiI, 99% had smooth cell bodies with Dogiel Type II morphology. Of these neurons, 70% projected in the longitudinal direction and the majority of them (65%) were located anally from the DiI application site, i.e., had ascending projections. Ascending neurons projected over significantly shorter distances than descending ones (3.1+/-0.5 mm vs. 4.6+/-1.2 mm, respectively; P<0.01). Of the labeled myenteric neurons, 98% were ChAT immunoreactive. Of these neurons, 78% were also immunoreactive for Calb and were preferentially ascending neurons. ChAT-immunoreactive but Calb-negative neurons did not have preferential projection. This study revealed the presence of two populations of myenteric neurons projecting to the mucosa of the guinea pig proximal colon. Morphological characteristics and neurochemical coding were suggestive for a putative sensory function for these neurons.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0302-766X
pubmed:author
pubmed:issnType
Print
pubmed:volume
287
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
119-25
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Projections and neurochemical coding of myenteric neurons innervating the mucosa of the guinea pig proximal colon.
pubmed:affiliation
Department of Physiology, School of Veterinary Medicine of Hannover, Bischofsholer Damm 15/102, D-30173 Hannover, Germany. mschem@win.tiho-hannover.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't