Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5-6
|
| pubmed:dateCreated |
1997-2-25
|
| pubmed:abstractText |
The expression of 5alpha-reductase type 1 and type 2 isoenzymes in hyperplastic human prostate tissue and several human prostate cell lines was investigated by Northern blot analyses, reverse transcription-polymerase chain reaction (RT-PCR), and enzyme activity. Separation of stroma and epithelium was confirmed histologically and only preparations with no apparent contamination were employed in the subsequent studies. Poly(A)+ RNA was isolated from stromal and epithelial fractions and analysed by Northern blot and RT-PCR. Inhibition of epithelial and stromal 5alpha-reductase activities by 17beta-N,N-diethylcarbamoyl-4-methyl-4-aza-5alpha-androstan- 3-one (4MA) was assessed using a range of concentrations between 10(-13) and 10(-5) M. Results from Northern blot analyses and RT-PCR showed that the prostate stroma expressed 5alpha-reductase type 1 and type 2 isoenzymes, whereas the prostate epithelium only expressed 5alpha-reductase type 1. This was consistent with biphasic inhibition of 5alpha-reductase activity by 4MA in stroma and monophasic inhibition in epithelium. Cultured epithelial cells derived from human prostate only expressed 5alpha-reductase type 1 and had Vmax and Km values that approximated the lower end of the range reported for surgically removed prostate epithelium. The foregoing data explains the disparate activities of 5alpha-reductase, previously reported, in stroma and epithelium. The differential localization of these isoenzymes in the prostate suggests that future therapy of androgen-sensitive disease may be more successful through the use of selective inhibitors of the different 5alpha-reductase isoenzymes.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/17-N,N-diethylcarbamoyl-4-methyl-4-a...,
http://linkedlifedata.com/resource/pubmed/chemical/Androgen Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Azasteroids,
http://linkedlifedata.com/resource/pubmed/chemical/Cholestenone 5 alpha-Reductase,
http://linkedlifedata.com/resource/pubmed/chemical/Dihydrotestosterone,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Oxidoreductases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0960-0760
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
59
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
397-404
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9010345-Aged,
pubmed-meshheading:9010345-Androgen Antagonists,
pubmed-meshheading:9010345-Azasteroids,
pubmed-meshheading:9010345-Blotting, Northern,
pubmed-meshheading:9010345-Cholestenone 5 alpha-Reductase,
pubmed-meshheading:9010345-Dihydrotestosterone,
pubmed-meshheading:9010345-Epithelium,
pubmed-meshheading:9010345-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:9010345-Humans,
pubmed-meshheading:9010345-Hyperplasia,
pubmed-meshheading:9010345-Isoenzymes,
pubmed-meshheading:9010345-Kinetics,
pubmed-meshheading:9010345-Male,
pubmed-meshheading:9010345-Middle Aged,
pubmed-meshheading:9010345-Oxidoreductases,
pubmed-meshheading:9010345-Polymerase Chain Reaction,
pubmed-meshheading:9010345-Prostate,
pubmed-meshheading:9010345-Prostatic Neoplasms,
pubmed-meshheading:9010345-RNA, Messenger,
pubmed-meshheading:9010345-Stromal Cells,
pubmed-meshheading:9010345-Tumor Cells, Cultured
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Characterization of 5alpha-reductase gene expression in stroma and epithelium of human prostate.
|
| pubmed:affiliation |
Department of Cancer Endocrinology, British Columbia Cancer Agency, Vancouver, Canada.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|