Linked Life Data

9009092

Source:http://linkedlifedata.com/resource/pubmed/id/9009092

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-2-24
pubmed:abstractText
When Friend murine erythroleukemia (F-MEL) cells are induced to differentiate by dimethylsulfoxide (DMSO), erythroid-specific genes are transcriptionally activated. The erythroid transcription factor NF-E2 is essential for enhancer activity of the globin locus control regions. NF-E2 functions as a heterocomplex consisting of a 45-kDa subunit (NF-E2p45) and a 18-kDa subunit (NF-E2p18). The larger subunit NF-E2p45 is tissue-restricted and is believed to play a role in globin gene expression in F-MEL cells. The expression of the smaller subunit NF-E2p18, which is a Maf family member (MafK), is cell type- and developmental stage-specific. We have investigated the possible role of NF-E2p18 in Friend erythroid differentiation by stably transfecting either sense and antisense p18 constructs into differentiation-sensitive 745A and partially defective-differentiation TFP10 cell lines. Overexpression of NF-E2p18 induced expression of globin transcripts in both cell lines and increased their sensitivity to erythroid differentiation when exposed to DMSO. Conversely, inhibition of p18 expression by antisense transcripts resulted in the inhibition of DMSO-induced differentiation in both cell lines. These results indicate that NF-E2p18 is necessary for globin expression in F-MEL cells and that it is the predominant gene of the Maf family involved in DMSO-induced erythroid differentiation. Moreover F-MEL clones overexpressing NF-E2p18 showed an increase in specific NF-E2 DNA-binding activity whereas this activity was decreased in clones expressing antisense p18. Finally, studies using transient transfection assays showed that p18 activated NF-E2 site-dependent transcription in F-MEL cells. These data suggest that NF-E2p18 can participate in DMSO-induced erythroid differentiation of F-MEL cells by enhancing NF-E2 activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0887-6924
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
273-80
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9009092-3T3 Cells, pubmed-meshheading:9009092-Animals, pubmed-meshheading:9009092-Cell Differentiation, pubmed-meshheading:9009092-Dimethyl Sulfoxide, pubmed-meshheading:9009092-Erythroid Precursor Cells, pubmed-meshheading:9009092-Erythropoiesis, pubmed-meshheading:9009092-Friend murine leukemia virus, pubmed-meshheading:9009092-Gene Expression Regulation, Leukemic, pubmed-meshheading:9009092-Globins, pubmed-meshheading:9009092-Leukemia, Erythroblastic, Acute, pubmed-meshheading:9009092-MafK Transcription Factor, pubmed-meshheading:9009092-Mice, pubmed-meshheading:9009092-Neoplasm Proteins, pubmed-meshheading:9009092-Neoplastic Stem Cells, pubmed-meshheading:9009092-Nuclear Proteins, pubmed-meshheading:9009092-Oligonucleotides, Antisense, pubmed-meshheading:9009092-Recombinant Fusion Proteins, pubmed-meshheading:9009092-Transcription, Genetic, pubmed-meshheading:9009092-Transcription Factors, pubmed-meshheading:9009092-Tumor Cells, Cultured
pubmed:year
1997
pubmed:articleTitle
NF-E2p18/mafK is required in DMSO-induced differentiation of Friend erythroleukemia cells by enhancing NF-E2 activity.
pubmed:affiliation
INSERM U268, Hôpital Paul Brousse, Villejuif, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't