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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1997-3-13
|
| pubmed:abstractText |
Cellulases are usually classified as endoglucanases and cellobiohydrolases, but the heterogeneity of cellulose, in terms of particle size and crystallinity, has always represented a problem for the biochemical characterization of the enzymes. The synthesis of a bifunctionalized tetrasaccharide substrate suitable for measuring cellulase activity by resonance energy transfer is described. The substrate, which carries a 5-(2-aminoethylamino)-1-naphthalenesulfonate group on the non-reducing end and an indolethyl group on the reducing end, was prepared from beta-lactosyl fluoride and indolethyl beta-cellobioside by a chemoenzymatic approach using the transglycosylating activity of endoglucanase I of Humicola insolens as the key step. The bifunctionalized substrate has been used for the determination of the catalytic constants of H. insolens endoglucanase I and cellobiohydrolases I and II; this substrate could be of general use to measure the kinetic constants of cellulases able to act on oligomers of degree of polymerization <5. The data also provide evidence that cellobiohydrolases I and II are able to degrade an oligosaccharide substrate carrying non-carbohydrate substituents at both ends.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
31
|
| pubmed:volume |
272
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2709-13
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9006908-Carbohydrate Conformation,
pubmed-meshheading:9006908-Carbohydrate Sequence,
pubmed-meshheading:9006908-Cellulase,
pubmed-meshheading:9006908-Energy Transfer,
pubmed-meshheading:9006908-Indicators and Reagents,
pubmed-meshheading:9006908-Kinetics,
pubmed-meshheading:9006908-Magnetic Resonance Spectroscopy,
pubmed-meshheading:9006908-Mitosporic Fungi,
pubmed-meshheading:9006908-Molecular Sequence Data,
pubmed-meshheading:9006908-Naphthalenesulfonates,
pubmed-meshheading:9006908-Oligosaccharides,
pubmed-meshheading:9006908-Spectrometry, Fluorescence,
pubmed-meshheading:9006908-Substrate Specificity
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
A bifunctionalized fluorogenic tetrasaccharide as a substrate to study cellulases.
|
| pubmed:affiliation |
Centre de Recherches sur les Macromolécules Végétales, F-38041 Grenoble cedex 9, France.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|