Linked Life Data

9005940

Source:http://linkedlifedata.com/resource/pubmed/id/9005940

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1997-2-13
pubmed:abstractText
We have used the ELISPOT method employing plastic ELISA plates without substrate in agar for the detection of single cells producing interferon-gamma (IFN-gamma) and interleukin-4 (IL-4). When using PBMC directly stimulated in the assay wells with T cell mitogens it was possible to measure production of human IFN-gamma at an earlier time point and with a higher sensitivity compared to conventional nitrocellulose plates. The plastic surface was not autostimulatory for IFN-gamma production, as seems to be the case for nitrocellulose surfaces. Compared to the use of nitrocellulose plates, the use of plastic ELISA plates is considerably cheaper and easier to perform. The increased sensitivity for cytokine detection, together with minimal autostimulatory properties of the detection surface, makes this method suitable for the detection of spontaneous low grade cytokine production from cells obtained in vivo.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
200
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
17-26
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
A comparison between ELISPOT methods for the detection of cytokine producing cells: greater sensitivity and specificity using ELISA plates as compared to nitrocellulose membranes.
pubmed:affiliation
Department of Rheumatology, Karolinska Hospital, Stockholm, Sweden.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't